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AAT Bioquest

FAQ

Posted on May 12, 2023
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR


Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 2, 2023
What are features of next generation sequencing?
DNADNA and RNADNA sequencingNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
What are the limitations of Sanger sequencing?
DNADNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
Which technique is used to visualize DNA following a PCR reaction?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Proteinpolyacrylamide geldna ladder


Posted on May 2, 2023
What factors should I consider before I use restriction enzymes to cut DNA into small fragments?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on May 2, 2023
How is a DNA ladder used in gel electrophoresis?
Agarose gel electrophoresisDNADNA and RNAGel ElectrophoresisNucleic AcidsNucleusPCRpolyacrylamide geldna ladder


Posted on April 27, 2023
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium


Posted on April 27, 2023
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on February 13, 2023
What are the advantages of PCR (polymerase chain reaction)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What are the similarities between Fluorophore and Chromophore?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)DNA and RNA QuantitationDyes by Functional GroupPCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on February 19, 2021
What functions do the dNTPs play in PCR?
PCRdATPdCTPdGTPdNTPsdTTP


Posted on January 21, 2021
What are the different types of PCR?
PCRPolymerase chain reactionTypes of PCR


Posted on July 15, 2020
6-ROX is predominately used as a reference dye for performing PCR detections, however, 6-ROX is very unstable compared to other rhodamine dyes. What is a better dye replacement?
Chemical ReagentsDNA and RNADNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What are the main steps of cloning?
DNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)Purification


Posted on June 22, 2020
What is the difference between PCR and molecular cloning regarding DNA replication?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
What are the functions of introns?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)RNA


Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What is the difference between transcription and reverse transcription?
DNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What is the melting curve analysis in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on June 22, 2020
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases


Posted on June 22, 2020
What is a sense strand (or coding strand)?
DNADNA and RNANucleic AcidsPCRPolymerase Chain Reaction (PCR)dsDNAssDNA


Posted on June 22, 2020
How do I choose primers for PCR?
PCRPCR primerPolymerase Chain Reaction (PCR)


Posted on June 22, 2020
Why should primers used in PCR have similar melting temperatures?
DNAPCRPCR primerPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What is the difference between melting temperature and annealing temperature?
DNAPCRPolymerase Chain Reaction (PCR)ssDNA


Posted on June 22, 2020
What are the advantages of Taq polymerase in PCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of polymerases used in PCR?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What are the three stages of a PRC process?
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of PCR technology?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How does SYBR Green work in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
How do hydrolysis probes work in qPCR? probes work?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of Taq polymerase?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on May 18, 2020
What are the 4 steps of PCR?
Polymerase chain reactionPCRPCR steps


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
How long does next-generation sequencing (NGS) take?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the difference between next-generation (NGS) and high-throughput sequencing (HTS)?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the function of DMSO in PCR?
Chemical ReagentsDMSOPCRPolymerase Chain Reaction (PCR)


Posted on May 9, 2020
What is the function of MgCl2 in PCR?
Chemical ReagentsDNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Polymerasesmagnesium


Posted on May 9, 2020
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer


Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on April 2, 2020
What is Sanger sequencing and what is it used for?
DNA sequencingGel ElectrophoresisPCRPolymerase Chain Reaction (PCR)electrophoresisDNA and RNA QuantitationDNA and RNAReverse Transcription PCR (RT-PCR)


Posted on February 4, 2020
What is the difference between PCR and real-time PCR?
PCRqPCR


Posted on February 4, 2020
What materials are needed for PCR?
PCRPolymerase chain reaction


Posted on February 4, 2020
What is the basic principle of PCR?
PCRPolymerase chain reaction


Posted on June 1, 2018
What is the working solution concentration for 6-ROX glycine?
PCR