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AAT Bioquest

Posted on August 23, 2023
What are the principles of chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purificationaffinity purification


Posted on April 18, 2023
How do I choose a method for protein purification?
Antibodies and Proteomics BioconjugationAffinity ChromatographyAntibody LabelingAntibody and Protein LabelingChromatographic techniquesProteinProtein Purification


Posted on August 12, 2020
What is protein purification?
Protein PurificationExtractionPrecipitationChromatographic techniques


Posted on June 22, 2020
How do I remove protein tags after purification?
ProteinProtein PurificationPurification


Posted on June 22, 2020
What is a His tag? How does it work?
Antibodies and ProteomicsAntibody and Protein LabelingProteinProtein Purification


Posted on June 22, 2020
What types of protein tags are commonly used?
Peptide and OligonucleotideProteinProtein PurificationPurification


Posted on June 1, 2020
What are theoretical plates in HPLC?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
Why is trifluoroacetic acid (TFA) used in reverse-phase chromatography for protein purification?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is the difference between nitrocellulose and PVDF membranes in western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What is protein A chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
Why are proteins transferred from gel to a membrane in western blot?
Gel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What is the difference between SDS-PAGE and western blot?
Antibodies and ProteomicsGel ElectrophoresisProtein PurificationWestern Blot


Posted on June 1, 2020
What kinds of interactions are used in affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is the difference between ion exchange chromatography and affinity chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
How do you choose a buffer for ion exchange chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
How do you do ion exchange chromatography?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
Why is high concentration of NaCl used in ion-exchange chromatography for elution?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is ion-exchange chromatography (IXC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is size-exclusion chromatography (SEC) used for in biochemistry?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is void volume in size exclusion chromatography (SEC)?
Affinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What are the exclusion limit and permeation limit in size-exclusion chromatography (SEC)?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on June 1, 2020
What is size-exclusion chromatography?
Antibodies and ProteomicsAffinity ChromatographyChromatographic techniquesProtein Purification


Posted on April 24, 2020
How can I increase the expression level of a protein?
Antibodies and ProteomicsAffinity ChromatographyAntibody and Protein LabelingChromatographic techniquesExtractionProteinProtein Purification


Posted on April 24, 2020
What is the difference between BCA and Micro BCA assay?
Antibodies and ProteomicsAffinity ChromatographyBCA assayBradford assayChromatographic techniquesExtractionProteinProtein PurificationProtein concentration