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AAT Bioquest

FAQ

Posted on May 2, 2024
Is there alternative techniques to Southern blotting?
DNA and RNA QuantitationDNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Sanger SequencingWestern Blot


Posted on May 2, 2024
What are the advantages of southern blot over PCR?
Cellular Structures and OrganellesDNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Western Blot


Posted on April 26, 2024
What should I do to optimize the bisulfite PCR conditions for amplifying bisulfite-treated material?
Cellular Structures and OrganellesDNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on March 18, 2024
What methods are used to detect miRNAs?
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What are the differences between real time PCR and nested PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What are the functions of MgCI2 in a PCR reaction?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
Why is MgCI2 used in the PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
When do I need to add some extra amount of MgCI2?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What is multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 28, 2024
What is the principle of multiplex PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on November 8, 2023
What are the basic principles of real-time PCR?
Gene Expression Analysis & GenotypingPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on November 8, 2023
What are the major differences between qPCR and conventional PCR?
PCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 29, 2023
What are molecular beacons used for?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
How does a molecular beacon work?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What is the importance of molecular beacons?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the advantages of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the limitations of molecular beacon probes?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
Why are MBs particularly suited for mRNA studies?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the strategies for minimizing MB false-positive signals in living cells?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the properties of molecular beacons that affect nucleic acid detection?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the widely used detection methods in quantitative PCR (Q-PCR)
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the steps of a molecular beacon RT-PCR assay?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyes


Posted on August 29, 2023
What are the best ways to store a molecular beacon preparation?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on August 29, 2023
What are the reasons for the low signal-to-background ratio after the synthesis and purification of a molecular beacon and how do I solve the problem?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationNucleic AcidsPolymerase Chain Reaction (PCR)PurificationReal-Time PCR (qPCR)


Posted on August 14, 2023
Why is thermostable DNA polymerase required for PCR?
DNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the applications of PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq polymerase do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do I do if my PCR isn't working?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What does Taq buffer do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What do forward and reverse primers do in PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the similarities between forward and reverse primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the steps involved in getting the reverse primer sequence?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
What are the guidelines should I follow when designing primers?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why is it necessary to purify PCR products?
DNA and RNA QuantitationNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on August 14, 2023
Why are quenchers used in real time PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use one-step as opposed to two-step qRT-PCR?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Tide Quencher Dyes


Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing


Posted on August 4, 2023
What are the generally used detection methods in real time PCR (qPCR)?
Antibodies and ProteomicsAntibody and Protein LabelingDNA and RNA QuantitationPCR Detection of Viral DNA/RNAReal-Time PCR (qPCR)


Posted on August 4, 2023
What’s the significance of ROX reference dye in real time PCR (qPCR)?
Chemical ReagentsCell Structures and OrganellsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on May 24, 2023
What are the common methods available to detect kinase activities?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA) Förster Resonance Energy Transfer (FRET)EnzymesFluorescenceProteinReal-Time PCR (qPCR)qPCR


Posted on May 20, 2023
Is magnesium chloride an acid or base?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Is magnesium chloride soluble in organic solvents?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on May 20, 2023
How does magnesium concentration affect PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the ideal concentration of MgCl2?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What is the mechanism of MgCl2 during PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
Why is isothermal amplification not as commonly used as PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
When should I use isothermal amplification instead of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the advantages of isothermal amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the differences between isothermal amplification and PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 20, 2023
What are the alternative methods of polymerase chain reaction (PCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing


Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What is the workflow of real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the methods used to measure the DNA application in qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
Should I use probe-based detection or dye-based detection for my qPCR analysis?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the factors I should consider to get accurate and reproducible results for my probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the advantages of probe-based real-time PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the principle of probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
How do I design primers for qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What is the gold standard for gene quantification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 12, 2023
What is the significance of the cycle threshold?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on May 12, 2023
What are the advantages of multiplexing techniques in real time PCR (qPCR)?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What factors should I consider when I choose fluorophore/quencher combinations for my multiplex qPCR reactions?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on May 12, 2023
What are the steps involved in probe-based qPCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR


Posted on March 1, 2023
What is the procedure of expression screening?
DNA and RNA QuantitationDNA and RNAGel ElectrophoresisPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Western Blot


Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor


Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 23, 2023
What are the methods used to measure and detect transcription?
DNA and RNA QuantitationDNA and RNANucleusRNAReal-Time PCR (qPCR)in situ hybridizationqPCR


Posted on February 17, 2023
What are the differences between Taq polymerase and DNA polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of Taq polymerase enzymes?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the optimal magnesium concentration for Taq polymerase?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)magnesium


Posted on February 17, 2023
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What is the typical concentration for dNTPs?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
Does PCR use DNA or RNA primers?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 17, 2023
What are the properties of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What are the steps of PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What are the components required for PCR reaction?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How should oligos be purified for PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
How does PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on February 13, 2023
What is PCR used for?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on January 18, 2023
How much DMSO do I add to PCR?
DMSODNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on September 26, 2022
What are the differences between qPCR and ddPCR?
Cell Structures and OrganellesDNA and RNA QuantitationNucleusPhysiological ProbesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on September 19, 2022
What is the purpose of preparing a master mix?
Cell Structures and OrganellesDNA and RNA QuantitationPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on February 9, 2022
What are the common applications of PCR?
Polymerase Chain Reaction (PCR)Coronavirus (SARS-CoV-2)Real-Time PCR (qPCR)DNA and RNA Quantitation


Posted on December 16, 2020
What is qPCR?
DNA and RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on December 5, 2020
What is reverse transcription PCR?
DNA and RNA QuantitationDNA and RNAPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on November 23, 2020
What is PCR amplification?
DNA and RNA QuantitationDNA and RNANucleic AcidsPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What is the difference between transcription and reverse transcription?
DNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the general steps of transcription?
DNA and RNA QuantitationDNA and RNAPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
Are all DNA molecules protein-coding?
DNADNA and RNANucleic AcidsPolymerase Chain Reaction (PCR)ProteinRNAReal-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)


Posted on June 22, 2020
What are the advantages of Taq polymerase in PCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of Taq polymerase?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
Are there different types of polymerases used in PCR?
DNADNA and RNA QuantitationEnzymesPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on June 22, 2020
What are the three stages of a PRC process?
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
What are the limitations of PCR technology?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How does SYBR Green work in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
How do hydrolysis probes work in qPCR? probes work?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)Types of PCR


Posted on June 22, 2020
What are the differences between the detection methods based on a fluorescent probe and an intercalating dye in qPCR?
DNADNA and RNA QuantitationPCRPeptide and OligonucleotidePolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)


Posted on June 22, 2020
How do you design a fluorescent hydrolysis probe in qPCR?
DNA and RNA QuantitationDNA and RNAReal-Time PCR (qPCR)qPCR


Posted on June 22, 2020
What is the melting curve analysis in qPCR?
DNADNA and RNA QuantitationPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)qPCR


Posted on June 22, 2020
Are there different types of fluorescent probes used in qPCR?
Peptide and Oligonucleotide LabelingDNA and RNA QuantitationDNA and RNAPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)


Posted on May 9, 2020
What is a next-generation sequencing (NGS) library?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencingqPCR


Posted on May 9, 2020
How pure does DNA have to be for next-generation sequencing (NGS)?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR


Posted on April 24, 2020
How can I determine if a protein has been knocked out?
Antibodies and ProteomicsProteinReal-Time PCR (qPCR)Western BlotqPCR


Posted on April 7, 2020
6-VIC, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. Is it also often used for labeling oligos?
Peptide and Oligonucleotide LabelingDNA sequencingPCRPhysiological ProbesPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)