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AAT Bioquest

FAQ

Posted on May 15, 2024
What are the limitations of spectral imaging technique?
Flow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 15, 2024
What is spectral imaging?
Flow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 8, 2024
Can I run multiplex immunohistochemistry (IHC) using fluorescent detection?
Antibodies and ProteomicsAntibody and Protein LabelingFluorescenceImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on April 10, 2024
What is image cytometry technique?
Chemical ReagentsCell Viability AssaysCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry


Posted on April 10, 2024
What are the advantages of flow cytometry?
Cellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry


Posted on April 10, 2024
What factors should I consider when designing intracellular flow experiments?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry


Posted on April 10, 2024
What are the applications of flow cytometry?
Cellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry


Posted on February 2, 2024
Are there any reasons for using flow cytometry to measure calcium flux?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Can I use the same calcium reagents for microscopy as I do for flow?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsMicroscopyPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What does baseline fluorescence level represent for flow assays?
Flow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on February 2, 2024
Is it possible to measure real time calcium flux with a flow cytometer?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Which calcium dyes work with flow cytometry lasers?
Calcium IndicatorsFlow Cytometry ReagentsFluoresceinsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Can I combine Fura Red with Fluo-3 or Fluo-4 for ratiometric analysis?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Should cells be equilibrated before a flow calcium assay?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What kinds of stimulants are used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What is the purpose of ionomycin in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What buffers should be used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What is the difference between negative control and baseline for flow cytometry calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Are there any substitutes for probenecid in calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid


Posted on February 2, 2024
What is the purpose of probenecid in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid


Posted on February 2, 2024
Is a wash step required for calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on January 16, 2024
What are the types of cell labeling?
FISHFlow Cytometry ReagentsMicroscopySpectral Flow Cytometryfluorescence microscopein situ hybridization


Posted on August 3, 2023
How are they characterized?
Cellular Structures and OrganellesSpectral Flow Cytometryexosomesflow cytometry


Posted on July 24, 2023
How is propidium iodide typically applied to cells?
Chemical ReagentsApoptosis and NecrosisCell Viability AssaysFlow Cytometry ReagentsImmunohistochemistry (IHC)Nucleic AcidsPropidium iodide (PI)Spectral Flow Cytometry


Posted on June 21, 2023
What are the principles of 5-CFDA-AM assay?
Chemical ReagentsCell Viability AssaysFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometry


Posted on June 9, 2023
What are the methods used to analyze cell death?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesDNAFlow Cytometry ReagentsPropidium iodide (PI)Spectral Flow Cytometrycell death


Posted on May 24, 2023
What types of cells can I sort?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the factors that affect the actual yield of the sorted cell samples?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What effect will the cell sorting process have on my cells' viability?
Chemical ReagentsCell ViabilityFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
What are the techniques of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryCell Sorting Fluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What is the significance of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting


Posted on May 24, 2023
How are cells separated?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What are the applications of cell sorting?
Chemical ReagentsFISHFlow Cytometry ReagentsFluorescenceImmunohistochemistry (IHC)Spectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
How do I prepare the samples for cell separation?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
Why should I use dead cell exclusion dye with my cell sorting experiment?
Flow Cytometry ReagentsFluorescenceLive or DeadSpectral Flow CytometryCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 24, 2023
What are the general categories of cell sorting techniques?
Flow Cytometry ReagentsFluorescenceSpectral Flow CytometrycentrifugeCell SortingFluorescence Activated Cell Sorting (FACS)


Posted on May 10, 2023
What are the applications of dextran-FITC conjugation?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsMicroscopySpectral Flow Cytometrydextranlive cell analysislive cell tracking


Posted on May 9, 2023
What advantages does FACS have over MACS?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
How do I choose the best cell separation method for my research?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
What are the key measures of performance for cell separation methods?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
What are the relationships between FACS and flow cytometry?
BioconjugationChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the applications of flow cytometry and FACS?
BioconjugationApoptosisCell Cycle AnalysisCell Proliferation AssaysChemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
How does cell sorting work?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the differences between magnetic-activated cell sorting and FACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the pros of MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the two types of magnetic cell separation?
Flow Cytometry ReagentsFluoresceinsFluorescenceSpectral Flow Cytometry


Posted on May 9, 2023
What are the mechanisms used to label cells in MACS?
Bioconjugation Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometry


Posted on April 18, 2023
What types of antibodies can be labeled using antibody labeling kits?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on April 18, 2023
What is the purpose of an antibody labeling kit?
Antibodies and Proteomics BioconjugationAntibody LabelingAntibody and Protein LabelingImmunohistochemistry (IHC)Spectral Flow Cytometry


Posted on January 26, 2023
How do I solve the dead cell issue?
Antibodies and Proteomics BioconjugationCell Viability AssaysFlow Cytometry ReagentsLive or DeadSpectral Flow Cytometry


Posted on January 26, 2023
Why are dead cells problematic in flow cytometry?
Antibodies and Proteomics BioconjugationCell Viability AssaysCellular ProcessesDNA and RNALive or DeadSpectral Flow Cytometry


Posted on January 13, 2023
What should I consider when choosing a fluorescence spectrometer?
Chemical ReagentsFluorescenceSpectral Flow Cytometryemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on December 15, 2022
What are the differences between absorbance and transmittance?
AbsorbanceBeer-Lambert LawMeasure absorbanceSpectral Flow Cytometry


Posted on November 14, 2022
How is GFP produced?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry


Posted on November 14, 2022
How is GFP detected?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry


Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
How do I dissolve a FITC-dextran?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
Is FITC soluble in water?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence


Posted on November 7, 2022
What color does 7-AAD fluoresce?
Cell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsNucleusSpectral Flow Cytometryflow cytometry


Posted on July 26, 2022
What is Cytokeratin 18?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingImmunohistochemistry (IHC)Spectral Flow CytometryWestern Blotflow cytometryimmunocytochemistry


Posted on November 15, 2021
What instruments are necessary to perform spectral flow cytometry?
Spectral Flow CytometryBioconjugationFlow Cytometry Reagents


Posted on November 11, 2021
Does spectral flow cytometry require signal subtraction?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on November 11, 2021
Who invented spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on November 11, 2021
What is the difference between conventional and spectral flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on November 11, 2021
What is full spectrum flow cytometry?
BioconjugationFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on October 30, 2020
What factors will affect absorbance?
AbsorbanceMeasure absorbanceSpectral Flow CytometryspectrophotometerBeer-Lambert Law


Posted on June 22, 2020
What are the three main components of a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
Why does the sample stream in flow cytometer need to be aligned in single file?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometer


Posted on June 22, 2020
How is the sample stream focused in a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What optics are used on a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometer lasersflow cytometry


Posted on June 22, 2020
What is spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What is the main difference between spectral flow cytometry and conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What is the difference between prisms and diffraction gratings in terms of light dispersion when they are used in spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What is limiting the applications of far-red and near-infrared fluorophores in conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
Why spectral flow cytometry is advantageous over conventional flow cytometry in multi-color experiments?
Flow Cytometry ReagentsFluorescencePE and APCSpectral Flow Cytometryflow cytometry


Posted on June 22, 2020
What is the difference between compensation and spectral unmixing?
FluorescenceSpectral Flow Cytometryflow cytometer


Posted on June 22, 2020
How does spectral flow cytometry manage autofluorescence?
FluorescenceSpectral Flow Cytometryautofluorescenceflow cytometry


Posted on June 22, 2020
What is a gate in flow cytometry?
Spectral Flow Cytometryflow cytometerflow cytometry


Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is fluorescence compensation in flow cytometry?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
How can I set up compensation for tandem dyes in flow cytometry?
Förster Resonance Energy Transfer (FRET)FRETFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is FSC and SSC in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is the general protocol for analysis of surface expression of a CD marker using flow cytometry?
Antibodies and ProteomicsCluster of Differentiation (CD Antibodies)ProtocolSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
Why does trypan blue quench FITC or GFP in flow cytometry?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What dyes are used in flow cytometry for cell viability testing?
Cell ViabilityCell Viability AssaysCell-Impermeable DNA dyesFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometrycell-impermeableflow cytometry


Posted on May 18, 2020
What is cell sorting in flow cytometry?
Antibodies and ProteomicsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is fluorescent activated cell sorting (FACS)?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
How is RNA isolated?
Antibodies and ProteomicsDNA and RNAExtractionFlow Cytometry ReagentsPhysiological ProbesPurificationRNASpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is the function of chloroform in RNA extraction?
Chemical ReagentsCellular Structures and OrganellesDNA and RNAFlow Cytometry ReagentsPurificationRNASpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
What is the difference between Side Scatter Pulse Width (SSC-w) and Side Scatter Pulse Height (SSC-h)?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometrymultiplex


Posted on May 9, 2020
Can mitochondrial tracking probes be used with flow cytometry?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsMitochondriaSpectral Flow Cytometryflow cytometrymitochondria membrane potential


Posted on May 9, 2020
What does FACS backgating show?
Antibodies and ProteomicsCellular ProcessesPhysiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 9, 2020
Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsImmunohistochemistry (IHC)PE and APCSpectral Flow Cytometry


Posted on May 9, 2020
What antibody concentration is recommended for intracellular staining using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingFlow Cytometry ReagentsIntracellular ProbesSpectral Flow Cytometryflow cytometry


Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex


Posted on May 9, 2020
How can I analyze natural killer cells using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)Spectral Flow Cytometryflow cytometry


Posted on April 24, 2020
What is the right DAPI concentration to use in a flow cytometry experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCell Cycle AssaysCell Structure and OrganellesCellular ProcessesDAPIFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on April 24, 2020
Can antibodies for flow cytometry be used for fluorescence microscopy?
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope


Posted on April 17, 2020
What is the role of sodium azide in flow cytometry?
Antibodies and Proteomics Chemical ReagentsFlow Cytometry ReagentsFluorescencePhysiological ProbesSpectral Flow Cytometry


Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent