FAQ
Posted on May 2, 2023
What would happen if I forgot to add the sample loading buffer?
Buffer Preparations and RecipesChemical Reagentsbuffer
What would happen if I forgot to add the sample loading buffer?
Buffer Preparations and RecipesChemical Reagentsbuffer
Posted on May 2, 2023
What are the components in the sample loading buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer
What are the components in the sample loading buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
What materials are needed to make a phosphate buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer
What materials are needed to make a phosphate buffer?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
Why is a phosphate buffer solution especially useful for biological applications?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Why is a phosphate buffer solution especially useful for biological applications?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
What criteria should I use to determine the type of lysis buffer required for an experiment?
Chemical ReagentsBuffer Preparations and Recipesbuffer
What criteria should I use to determine the type of lysis buffer required for an experiment?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
What are the common lysis buffers for general protein extraction?
Chemical ReagentsBuffer Preparations and Recipesbuffer
What are the common lysis buffers for general protein extraction?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
How do I determine the concentration of an unknown acid or base solution?
Chemical ReagentsBuffer Preparations and Recipesbuffer
How do I determine the concentration of an unknown acid or base solution?
Chemical ReagentsBuffer Preparations and Recipesbuffer
Posted on April 26, 2023
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
What are the most common buffers used for agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
Posted on April 26, 2023
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
When should I use a TBE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
Posted on April 26, 2023
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
When should I use a TAE buffer for gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
Posted on April 14, 2023
How do I prepare a solution of a specific concentration?
Buffer Preparations and RecipesStock SolutionWorking Solutionbuffer
How do I prepare a solution of a specific concentration?
Buffer Preparations and RecipesStock SolutionWorking Solutionbuffer
Posted on March 28, 2023
Are solutions homogeneous?
Chemical ReagentsStock SolutionWorking Solutionbuffer
Are solutions homogeneous?
Chemical ReagentsStock SolutionWorking Solutionbuffer
Posted on March 17, 2023
What are the different types of mixture based on the size of the particles involved?
Chemical ReagentsBuffer Preparations and RecipesStock Solutionbuffer
What are the different types of mixture based on the size of the particles involved?
Chemical ReagentsBuffer Preparations and RecipesStock Solutionbuffer
Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
Posted on September 30, 2020
How do I lyse cells?
Cell Cycle AnalysisCellular Structures and OrganellesDNA and RNAbuffercell lysis
How do I lyse cells?
Cell Cycle AnalysisCellular Structures and OrganellesDNA and RNAbuffercell lysis
Posted on July 22, 2020
What components are needed in cell culture media?
Cell Cultureamino acid sequencebuffer
What components are needed in cell culture media?
Cell Cultureamino acid sequencebuffer
Posted on June 1, 2020
What is the difference between PAGE and SDS-PAGE?
Buffer Preparations and RecipesGel ElectrophoresisGel Electroporesisbufferdetergent
What is the difference between PAGE and SDS-PAGE?
Buffer Preparations and RecipesGel ElectrophoresisGel Electroporesisbufferdetergent
Posted on June 1, 2020
What is the function of electrophoresis buffer?
DNA and RNA QuantitationDNA and RNAGel Electrophoresisbuffer
What is the function of electrophoresis buffer?
DNA and RNA QuantitationDNA and RNAGel Electrophoresisbuffer
Posted on May 18, 2020
What does EDTA do in agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisChelatorEDTAGel Electrophoresisbuffer
What does EDTA do in agarose gel electrophoresis?
Chemical ReagentsAgarose gel electrophoresisChelatorEDTAGel Electrophoresisbuffer
Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent
Posted on May 9, 2020
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer
For primer dilution in PCR, should I use water or TE buffer?
Chemical ReagentsBuffer Preparations and RecipesPCRPolymerase Chain Reaction (PCR)buffer
Posted on April 24, 2020
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence
Posted on April 24, 2020
What is the difference between staining buffer and FACS buffer?
Chemical ReagentsBSABuffer Preparations and RecipesEDTAImmunohistochemistry (IHC)PBSbuffer
What is the difference between staining buffer and FACS buffer?
Chemical ReagentsBSABuffer Preparations and RecipesEDTAImmunohistochemistry (IHC)PBSbuffer
Posted on April 24, 2020
How do you dilute primary antibodies from 1:200 to 1:1000?
Chemical ReagentsBuffer Preparations and RecipesCalculating ConcentrationIHCImmunohistochemistry (IHC)Serial Dilutionbuffer
How do you dilute primary antibodies from 1:200 to 1:1000?
Chemical ReagentsBuffer Preparations and RecipesCalculating ConcentrationIHCImmunohistochemistry (IHC)Serial Dilutionbuffer
Posted on April 24, 2020
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer
What is the difference between bicarbonate and PBS as a coating buffer in ELISA assay?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)Buffer Preparations and RecipesELISAPBSbuffer
Posted on April 17, 2020
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization
Does PBS buffer have any advantage over SSC buffer?
Bioconjugation Chemical ReagentsBuffer Preparations and RecipesDNA and RNAFISHPhysiological Probesbufferfluorescence microscopein situ hybridization
Posted on April 17, 2020
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer
What is the actual mechanism involved in blocking a nitrocelluolse membrane ?
Antibodies and Proteomics Bioconjugation Chemical ReagentsBSABuffer Preparations and Recipesbovine serum albuminbuffer
Posted on April 17, 2020
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer
What is the benefit of using TN buffer (10 mM Tris, 400 mM Nacl, pH 7.5) in the RNA extraction?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesDNA and RNAExtractionRNAbuffer
Posted on April 17, 2020
How does PBS clean cells?
Chemical ReagentsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)PBSbuffer
How does PBS clean cells?
Chemical ReagentsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)PBSbuffer
Posted on April 17, 2020
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer
What is the function of adding BSA as buffer in Biotin-Streptavidin system?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBSABiotin and StreptavidinBuffer Preparations and Recipesbovine serum albuminbuffer
Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer
Posted on April 17, 2020
Does PBS buffer have any effect on cell adhesion?
Buffer Preparations and RecipesPBSadherent cellsbuffer Chemical Reagents
Does PBS buffer have any effect on cell adhesion?
Buffer Preparations and RecipesPBSadherent cellsbuffer Chemical Reagents
Posted on April 17, 2020
Best hybridization buffer for DNA Hybridization?
Buffer Preparations and Recipesbuffer
Best hybridization buffer for DNA Hybridization?
Buffer Preparations and Recipesbuffer
Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent
Posted on April 17, 2020
What is the best blocking buffer for immunofluorescence ?
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence
What is the best blocking buffer for immunofluorescence ?
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence
Posted on April 17, 2020
Why do we use Citrate buffer in Antigen retrieval step during IHC?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)buffer Chemical ReagentsBuffer Preparations and Recipes
Why do we use Citrate buffer in Antigen retrieval step during IHC?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)buffer Chemical ReagentsBuffer Preparations and Recipes
Posted on April 17, 2020
When should DTT be added in tissue lysis?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesPhysiological Probesbuffer
When should DTT be added in tissue lysis?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesPhysiological Probesbuffer
Posted on April 17, 2020
Should I use cold or warm PBS washes when performing protein extraction?
Buffer Preparations and RecipesPBSbuffer Antibodies and ProteomicsEnzymesExtractionProtein
Should I use cold or warm PBS washes when performing protein extraction?
Buffer Preparations and RecipesPBSbuffer Antibodies and ProteomicsEnzymesExtractionProtein
Posted on April 17, 2020
What is blocking buffer in IHC?
Antibodies and ProteomicsBSAImmunohistochemistry (IHC)PBSbuffer Chemical ReagentsIHC
What is blocking buffer in IHC?
Antibodies and ProteomicsBSAImmunohistochemistry (IHC)PBSbuffer Chemical ReagentsIHC
Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent
Posted on April 17, 2020
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer
What's the role of BSA/Milk as blocking agents??
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)bovine serum albuminbuffer
Posted on April 17, 2020
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent
Posted on April 17, 2020
What is the purpose of a buffered solution used for RNase treatment?
Chemical ReagentsBuffer Preparations and RecipesDNA PurityDNA and RNAEnzymesNucleic Acidsbuffer
What is the purpose of a buffered solution used for RNase treatment?
Chemical ReagentsBuffer Preparations and RecipesDNA PurityDNA and RNAEnzymesNucleic Acidsbuffer
Posted on April 17, 2020
What % of BSA to use for blocking step?
Antibodies and Proteomics Chemical ReagentsBSABuffer Preparations and RecipesPhysiological Probesbovine serum albuminbuffer
What % of BSA to use for blocking step?
Antibodies and Proteomics Chemical ReagentsBSABuffer Preparations and RecipesPhysiological Probesbovine serum albuminbuffer
Posted on February 19, 2020
Why are my NAD/NADH ratio readings erratic when compared to my standard solution?
AmpliteControlsNAD/NADH RatioNADH AssaySerial DilutionStock SolutionWorking Solutionbuffercaspaseenzymenicotinamide adenine dinucleotide
Why are my NAD/NADH ratio readings erratic when compared to my standard solution?
AmpliteControlsNAD/NADH RatioNADH AssaySerial DilutionStock SolutionWorking Solutionbuffercaspaseenzymenicotinamide adenine dinucleotide
Posted on February 19, 2020
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer