calcium | AAT Bioquest

Posted on November 6, 2020
What are second messengers?
Cell SignalingSecond MessengerCalcium Indicatorcalcium

Posted on February 19, 2020
What are possible reasons for inconsistent Fluo-4 AM loading?
AM esterCalcium dyesGreen Fluorescencecalciumcalcium indicatorcell linescell-permeable probescell culturedegree of labelingfluo-4

Posted on December 10, 2019
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
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Posted on June 26, 2019
What is the working mechanism of Ionomycin?
calciumcalcium indicatorGPCRcalcium fluxcalcium mobilityIonomycinionophore

Posted on June 26, 2019
Why do CHO cells require the use of probenecid in calcium assays?
calcium fluxcalcium indicatorcalciumcell assayprobenecidorganic anion transporterCHOGPCR

Posted on June 13, 2019
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR

Posted on June 13, 2019
What is the pH value of your probenecid solution (Cat# 20062)?
calciumprobenecidcalbryte 520calcium indicator

Posted on June 13, 2019
What is the best way to quantitate Cyclic ADP-Ribose (cADPR)?
NADCa2+calciumcell assayfluorimetriccalcium mobilitysecond messengerfluorescent plate reader

Posted on June 12, 2019
Can I fix the cells before or after addition of Calcium detection dyes?
calcium indicatorcalciumcalcium fluxCa2+GPCRcalbryteCalcium dyes

Posted on April 2, 2019
When performing a calcium flux assay in living cells do probes such as Fluo 8, Cal 520 or Calbryte 520 require a washing step after incubation, similar to that of Fluo-4?
calcium

Posted on April 1, 2019
What is the molar extinction coefficient of RNA?
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Posted on April 1, 2019
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
calciumcalcium indicator

Posted on April 1, 2019
What is the difference between EDTA and EGTA ?
Ca2+Mg2+EDTAEGTAcalciummagnesium

Posted on April 1, 2019
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator

Posted on April 1, 2019
Which fluorescent calcium indicators can be used to directly measure lysosomal calcium concentration?
calciumdextranlysosomal calcium

Posted on April 1, 2019
What is the concentration of calcium inside cells?
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Posted on September 26, 2018
What are the three common chemical forms of calcium indicators? What is the difference between salts, dextran conjugates, and acetoxymethyl (AM) esters?
Ca2+calciumcalcium indicatorcalcium mobilitycalcium fluxGPCRcalbrytecalbryte 520fluo-4

Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathway?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator

Posted on July 9, 2018
Are there any secondary messengers involved in the lysosomal calcium signaling pathways?
Ca2+NAADPcalciumlysosomecalcium fluxcalcium mobilitycalcium indicatorlysosomal calciumsecond messengercalcium concentrationfluorescent calcium indicator

Posted on July 5, 2018
What products would you recommend for monitoring calcium concentrations in the ER, mitochondria and cytoplasm?
GPCRcalciumcytoplasmcalcium fluxcalbryte 520mitochondriafluorescent calcium indicatorEndoplasmic Reticulum (ER)

Posted on June 15, 2018
Do you offer any calcium indicators for plant cells?
Ca2+GPCRcalciumplant cellscalcium fluxcalbryte 520fluorescent calcium indicator

Posted on April 9, 2018
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
GPCRcalciumcalcium fluxcalcium mobilitycalcium indicatorEndoplasmic Reticulum (ER)

Posted on February 24, 2018
What is HHBS?
Ca2+calciumcell assaycalcium fluxcalcium indicatormembrane potential