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AAT Bioquest

Posted on February 13, 2023
What are the common PCR additives?
BSADMSODNA and RNA QuantitationDNA and RNAPolymerase Chain Reaction (PCR)bovine serum albumindetergentmagnesium


Posted on September 19, 2022
What is SDS-PAGE?
Chemical ReagentsAgarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)detergentpolyacrylamide gel


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on August 12, 2020
What are the best ways to exchange detergents for membrane protein studies?
CMCDialysisdetergentmembrane proteins


Posted on June 22, 2020
How does (sodium dodecyl sulfate) SDS denature a protein?
Antibodies and ProteomicsProteindetergent


Posted on June 1, 2020
What is the difference between PAGE and SDS-PAGE?
Buffer Preparations and RecipesGel ElectrophoresisGel Electroporesisbufferdetergent


Posted on June 1, 2020
What is the role/function of SDS in SDS-PAGE?
Chemical Reagentsdetergent


Posted on May 9, 2020
What is the best method for permeabilizing cells?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent


Posted on April 17, 2020
Is agitation necessary during IHC antibody incubation?
Antibodies and ProteomicsBuffer Preparations and RecipesIHCImmunohistochemistry (IHC)Permeabilizationbufferdetergent


Posted on April 17, 2020
What is the advantage or dissadvantage of having Tween 20 in flow cytometry wash and staining buffers?
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesFlow Cytometry ReagentsSpectral Flow Cytometrybufferdetergent


Posted on April 17, 2020
What is the role of Tween-20 in antigen retrieval buffer?
Immunohistochemistry (IHC)detergent Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesIHCbuffer


Posted on April 17, 2020
Does adding triton with the primary antibody hinder antigen detection in immunohistochemistry?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Primary Antibodies Chemical ReagentsBuffer Preparations and RecipesIHCbufferdetergent


Posted on September 26, 2018
Why are the aldehyde and peroxide contents of a detergent important?
aldehydeperoxidedetergent


Posted on July 16, 2018
What is the aggregation number in chemistry?
Aggregation numberdetergent


Posted on July 16, 2018
What is the cloud point in chemistry?
Cloud pointdetergent


Posted on July 16, 2018
What is the critical micelle concentration (CMC) in chemistry?
Critical micelle concentrationCMCdetergent


Posted on July 16, 2018
What is the average micellar weight?
relative micellar massaverage micellar weightdetergent