AAT Bioquest

Posted on February 27, 2020
Do double reciprocal plot (1/Vo versus 1/[S]) work the same way as an IC50 plot and an enzyme kinetic plot?
IC50IC50 CalculatorDynamic Rangeenzymereciprocal plotenzyme kinetic plot

Posted on February 19, 2020
What is the primary function of β-galactosidase?
AmpliteenzymeCell Metabolismbeta-galactosidase

Posted on February 19, 2020
Why are my NAD/NADH ratio readings erratic when compared to my standard solution?
AmpliteControlsNAD/NADH RatioNADH AssaySerial DilutionStock SolutionWorking Solutionbuffercaspaseenzymenicotinamide adenine dinucleotide

Posted on February 19, 2020
My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?
AmpliteNADNAD/NADH RatioNADHNADPNADP/NADPH ratioNADPHcell linesenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphateNADH Assay

Posted on February 19, 2020
Why is my NADH assay showing unreasonably high absorbance readings?
AbsorbanceCell AssayColorimetricNADH AssayNADNADHNADPNADPHStock Solutionbufferenzymenicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphatespectrophotometer

Posted on November 9, 2019
What is the role of the LacZ enzyme?
E. colienzymelac Operonβ-galactosidaseProtein