flow cytometry | AAT Bioquest

Posted on September 11, 2024
How do I remove cellular debris from my mixture?
Apoptosis and NecrosisCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsFluorescence Activated Cell Sorting (FACS)flow cytometry

Posted on August 3, 2023
How are they characterized?
Cellular Structures and OrganellesSpectral Flow Cytometryexosomesflow cytometry

Posted on May 24, 2023
What are the factors that affect the actual yield of the sorted cell samples?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
What effect will the cell sorting process have on my cells' viability?
Chemical ReagentsCell ViabilityFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
What are the techniques of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryCell Sorting Fluorescence Activated Cell Sorting (FACS)

Posted on May 24, 2023
What is the significance of cell sorting?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometerflow cytometryFluorescence Activated Cell Sorting (FACS)Cell Sorting

Posted on May 24, 2023
How do I prepare the samples for cell separation?
Chemical ReagentsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometryCell SortingFluorescence Activated Cell Sorting (FACS)

Posted on November 14, 2022
How is GFP produced?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry

Posted on November 14, 2022
How is GFP detected?
Antibodies and ProteomicsGreen FluorescenceProteinSpectral Flow Cytometryflow cytometry

Posted on November 7, 2022
What color does 7-AAD fluoresce?
Cell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesFlow Cytometry ReagentsNucleusSpectral Flow Cytometryflow cytometry

Posted on July 26, 2022
What is Cytokeratin 18?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody LabelingImmunohistochemistry (IHC)Spectral Flow CytometryWestern Blotflow cytometryimmunocytochemistry

Posted on June 22, 2020
What is the difference between prisms and diffraction gratings in terms of light dispersion when they are used in spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What are the three main components of a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
How is the sample stream focused in a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What optics are used on a flow cytometer?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometer lasersflow cytometry

Posted on June 22, 2020
What is spectral flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What is the main difference between spectral flow cytometry and conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
What is limiting the applications of far-red and near-infrared fluorophores in conventional flow cytometry?
Flow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
Why spectral flow cytometry is advantageous over conventional flow cytometry in multi-color experiments?
Flow Cytometry ReagentsFluorescencePE and APCSpectral Flow Cytometryflow cytometry

Posted on June 22, 2020
How does spectral flow cytometry manage autofluorescence?
FluorescenceSpectral Flow Cytometryautofluorescenceflow cytometry

Posted on June 22, 2020
What is a gate in flow cytometry?
Spectral Flow Cytometryflow cytometerflow cytometry

Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence

Posted on June 1, 2020
What are the common methods for bacteria counting?
Flow Cytometry ReagentsFluorescenceGram Stainingbacteriaflow cytometryfluorescence imagingfluorescence microscope

Posted on June 1, 2020
How can I set up compensation for tandem dyes in flow cytometry?
Förster Resonance Energy Transfer (FRET)FRETFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is fluorescence compensation in flow cytometry?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
What is spillover in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometryflow cytometry

Posted on June 1, 2020
How does BrdU labeling work?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
How long is the duration of each phase in a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
What is the mitosis phase in a cell cycle and what are the phases of mitosis phase?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
What are cell cycle and cell cycle phases?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on June 1, 2020
What is cell division and types?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry

Posted on May 18, 2020
What is the general protocol for analysis of surface expression of a CD marker using flow cytometry?
Antibodies and ProteomicsCluster of Differentiation (CD Antibodies)ProtocolSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is FSC and SSC in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is the difference between Side Scatter Pulse Width (SSC-w) and Side Scatter Pulse Height (SSC-h)?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometrymultiplex

Posted on May 18, 2020
What is the function of chloroform in RNA extraction?
Chemical ReagentsCellular Structures and OrganellesDNA and RNAFlow Cytometry ReagentsPurificationRNASpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
How is RNA isolated?
Antibodies and ProteomicsDNA and RNAExtractionFlow Cytometry ReagentsPhysiological ProbesPurificationRNASpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is fluorescent activated cell sorting (FACS)?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsPhysiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What is cell sorting in flow cytometry?
Antibodies and ProteomicsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 18, 2020
What dyes are used in flow cytometry for cell viability testing?
Cell ViabilityCell Viability AssaysCell-Impermeable DNA dyesFlow Cytometry ReagentsFluorescenceSpectral Flow Cytometrycell-impermeableflow cytometry

Posted on May 18, 2020
Why does trypan blue quench FITC or GFP in flow cytometry?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesFlow Cytometry ReagentsSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
Can mitochondrial tracking probes be used with flow cytometry?
Chemical ReagentsCellular ProcessesFlow Cytometry ReagentsMitochondriaSpectral Flow Cytometryflow cytometrymitochondria membrane potential

Posted on May 9, 2020
Which markers can be used to differentiate between human monocytes and macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)flow cytometryimmunofluorescence

Posted on May 9, 2020
What stain should I use to analyze apoptosis in flow cytometry?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesFlow Cytometry ReagentsFluoresceinsannexin Vflow cytometry

Posted on May 9, 2020
What markers can I use for investigating microglial activation in flow cytometry?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)flow cytometry

Posted on May 9, 2020
What are the best methods to identify necrotic cells?
Antibodies and ProteomicsApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesDehydrogenaseEnzymesFlow Cytometry Reagentsflow cytometry

Posted on May 9, 2020
What does FACS backgating show?
Antibodies and ProteomicsCellular ProcessesPhysiological ProbesSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
What antibody concentration is recommended for intracellular staining using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingFlow Cytometry ReagentsIntracellular ProbesSpectral Flow Cytometryflow cytometry

Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex

Posted on May 9, 2020
How can I analyze natural killer cells using flow cytometry?
Antibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsIHCImmunohistochemistry (IHC)Spectral Flow Cytometryflow cytometry

Posted on April 24, 2020
Can antibodies for flow cytometry be used for fluorescence microscopy?
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope

Posted on April 17, 2020
Why should a cell die or undergo apoptosis?
ApoptosisApoptosis and NecrosisCell ViabilityCellular Processescell deathFlow Cytometry ReagentsPhysiological Probesflow cytometry

Posted on February 19, 2020
How do I determine transfection efficiency using a flow cytometer?
ControlsTransfectionflow cytometerflow cytometrylive cell analysis

Posted on February 19, 2020
Do you have a suggested protocol for cell transfection?
DNA PurityTransfectionflow cytometrylive cell analysis

Posted on August 21, 2019
What are common laser lines used in flow cytometry?
flow cytometryflow cytometer lasers iFluor dyes

Posted on June 13, 2019
Does Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit support multiplex assays for detection of mitochondrial superoxide?
cell-permeable probesflow cytometryfluorescence microscopelive cell trackingmitochondriasuperoxide

Posted on June 12, 2019
Do you have fixable live cell staining assay kits?
Cell CytotoxicityCell ViabilityMultidrug Resistance (MDR1/P-gp, MRP1)flow cytometryfluorescence imagingfluorescence microplate readerfluorescent microscopefluorescent plate readerlive cell assay

Posted on June 12, 2019
Can I use any of your Cell Meter™ Autophagy Assay Kits with a flow cytometer?
absorbancefluorescent plate readerAutophagyflow cytometry

Posted on June 12, 2019
Can I add MitoROS dyes to my cell growth media?
ROScell assayflow cytometry

Posted on June 11, 2019
Apoptosis assay selection for GFP and RFP transfected tumor cells?
apoptosisfluorescent plate readercell assayflow cytometrytumor

Posted on April 2, 2019
How to measure intracellular pH using flow cytometry?
Working Solutioncell-permeable probesflow cytometerflow cytometrypH probes

Posted on April 2, 2019
Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?
flow cytometerflow cytometryfluorescence imagingfluorescence microplate readerfluorescence microscopefluorescent plate reader

Posted on January 30, 2018
Why should I perform a compensation correction when using JC-10 in flow cytometry assays to quantitate mitochondrial membrane potential?
mitochondriamitochondria membrane potentialflow cytometrycompensation correctionJC-10JC-1J-aggregateJ-monomer