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AAT Bioquest

FAQ

Posted on August 16, 2023
What are the requirements of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the steps involved in DNA based microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the principles of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the applications of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the differences between DNA microarray and RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the pros of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the cons of DNA microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the cons of RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the pros of RNA sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the differences between karyotype and chromosome microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the similarities between karyotype and chromosome microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the types of microarrays based on the mode of preparation?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the types of microarrays based on the probes used?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the limitations of karyotyping?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What can CMAs not detect?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the different types of chromosome studies?
DNA and RNA QuantitationFISHNucleic AcidsNucleusgene sequencingin situ hybridization


Posted on August 16, 2023
What are the differences between microarray and next generation sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the similarities between microarray and next generation sequencing?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the challenges of chromosomal microarrays?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What are the benefits of SNPs?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What types of disorders can chromosome microarray analysis diagnose?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 16, 2023
What is the general workflow of SNP microarray?
DNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 14, 2023
When should I use dPCR to quantify NGS libraries?
DNA and RNA QuantitationPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Tide Quencher Dyesgene sequencing


Posted on August 10, 2023
What is the basic principle behind RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 10, 2023
How does RNA-Seq contribute to the understanding of gene expression patterns?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What types of RNA can be analyzed using RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What sequencing technologies are commonly used for RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
How much sequencing depth is required for RNA-seq experiments?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What method can I use to remove ribosomal RNA?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What are the different types of RNA-seq?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What are the advantages of long-read sequencing?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 10, 2023
What main parameters should I consider when setting up a new RNA-seq experiment?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusgene sequencing


Posted on August 9, 2023
How can I access and analyze a publicly available RNA-seq database?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
How do I prepare an RNA sequencing library?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are the challenges of RNA-Seq technology?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
Why is RNA-seq considered superior to microarrays?
DNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on August 9, 2023
What are some challenges and solutions in single cell RNA-Seq data analysis?
Cellular Structures and OrganellesDNA and RNA QuantitationNucleic AcidsNucleusPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing


Posted on May 12, 2023
What's the significance of reference genes?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 2, 2023
What are features of next generation sequencing?
DNADNA and RNADNA sequencingNucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on May 2, 2023
What are the limitations of Sanger sequencing?
DNADNA and RNANucleic AcidsNucleusPCRPolymerase Chain Reaction (PCR)Sanger Sequencinggene sequencing


Posted on April 19, 2023
What is the basic structure of a gene?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusTransfectionTransfection Reagentsgene sequencing


Posted on April 19, 2023
What are the functions of genes?
Cellular Structures and OrganellesDNA and RNA QuantitationDNA and RNANucleic AcidsNucleusgene sequencing


Posted on September 19, 2022
Why is SDS used in protein gel electrophoresis?
Agarose gel electrophoresisDNA and RNADNA sequencingGel ElectrophoresisPolymerase Chain Reaction (PCR)Western Blotdetergentgene sequencingpolyacrylamide gel


Posted on September 16, 2020
What is gene sequencing?
DNAdna sequencinggene sequencing


Posted on July 22, 2020
What types of reversible terminators are available for next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What methods are used in next-generation sequencing (NGS) to amplify templates?
DNADNA and RNADNA sequencingPolymerase Chain Reaction (PCR)gene sequencing


Posted on July 22, 2020
How does bridge amplification work in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by synthesis approach in next-generation sequencing (NGS)?
Cellular processesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is sequencing by ligation approach in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is a reversible terminator used in next-generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the major platforms for next generation sequencing (NGS)?
Cellular ProcessesDNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is the general procedure of Illumina sequencing?
Cellular ProcessesDNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What is pyrosequencing?
DNADNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How does pyrosequencing work?
DNADNA and RNA QuantitationDNA and RNADNA sequencingEnzymesgene sequencing


Posted on July 22, 2020
What is third-generation sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
DNADNA and RNA QuantitationDNA and RNADNA sequencinggene sequencing


Posted on July 22, 2020
How do antisense oligonucleotides work in gene silencing?
DNADNA and RNANucleic AcidsPeptide and OligonucleotideRNAgene sequencing


Posted on July 22, 2020
What is the difference between gene silencing and gene knockout?
DNADNA and RNANucleic AcidsRNAgene sequencing


Posted on June 22, 2020
What are introns?
DNADNA and RNADNA sequencingNucleic AcidsProteinRNAgene sequencing


Posted on May 9, 2020
Can next-generation sequencing (NGS) sample preparation be automated?
DNA and RNADNA sequencingPhysiological Probesgene sequencing


Posted on May 9, 2020
What is a next-generation sequencing (NGS) library?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencingqPCR


Posted on May 9, 2020
How pure does DNA have to be for next-generation sequencing (NGS)?
DNA and RNADNA sequencingPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)gene sequencing


Posted on May 9, 2020
How does next-generation sequencing (NGS) work?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
How long does next-generation sequencing (NGS) take?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
What is the difference between next-generation (NGS) and high-throughput sequencing (HTS)?
DNA and RNADNA sequencingNucleic AcidsPCRPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Is next-generation sequencing (NGS) better than Sanger sequencing?
DNA and RNA QuantitationDNA and RNADNA sequencingNucleic AcidsPolymerase Chain Reaction (PCR)gene sequencing


Posted on May 9, 2020
Why am I getting multiple bands for my PCR results?
DNA and RNADNA sequencingPCRPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)gene sequencing


Posted on May 9, 2020
What is next-generation sequencing (NGS)?
Chemical ReagentsDNA and RNADNA sequencingPhysiological ProbesPolymerase Chain Reaction (PCR)gene sequencing