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AAT Bioquest

FAQ

Posted on January 22, 2020
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader


Posted on January 22, 2020
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader


Posted on April 2, 2019
How does the Amplite® Fluorimetric Glutathione GSH/GSSG Ratio assay work? What is the assay actually quantifying?
GSSGGSHGSH/GSSG ratiocell-permeable probesfluorimetricglutathione


Posted on August 15, 2018
Is there a problem with my calibration curve of GSH and Total GSH for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit? Why does my standard curve not make sense?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader


Posted on August 15, 2018
Can I use Triton X-100 as my lysis buffer for Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence* (Cat# 10056)? Can I use X buffer to lysate/prepare my samples?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader


Posted on August 15, 2018
How do I prepare my sample for use in Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*? Can this assay be used for X cells?
GSHGSSGGSH/GSSG ratioglutathionefluorimetricfluorescence microplate reader


Posted on August 10, 2018
Why is the Total GSH standard solution concentration equal to twice that of the GSSG standard solution concentration? Why is [GSSG] equal to ([Total GSH] - [GSH]) DIVIDED by 2?
GSHGSSGglutathioneGSH/GSSG ratiofluorimetricfluorescent plate reader