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AAT Bioquest

FAQ

Posted on June 20, 2024
What are the applications of CLIP-tag?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingPhysiological Probesimmunofluorescence


Posted on May 8, 2024
Can I use a conjugated primary antibody in my immunofluorescence experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 10, 2024
What should I do if I have a high background (uniform distribution) in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What should I do to get optimal transfer results?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 10, 2024
What are the variables that need to be considered when optimizing protein transfer?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingWestern Blotimmunofluorescence


Posted on April 10, 2024
What roles do SDS and alcohol play in the western blot transfer step?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the different types of transfer methods in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
How do I validate the transfer?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 10, 2024
Why is the data produced with a western blot typically considered to be semi-quantitative?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the applications of southern blotting?
Antibodies and Proteomics BioconjugationDNA and RNA QuantitationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the principles of southern blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the principles of western blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the applications of western blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What do I need for my western blot membrane blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the disadvantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the advantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the differences between nitrocellulose membrane and nylon membrane?
Antibodies and ProteomicsPhysiological ProbesSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
How do I optimize my western blot experiments?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What are the common causes of an unexpected size of western blot band?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on April 10, 2024
What should I take into consideration before I strip and reprobe my western blotting experiment?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence


Posted on March 11, 2024
Why do we need controls for immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on March 11, 2024
What are the different types of control for immunofluorescence?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingControlsimmunofluorescence


Posted on March 11, 2024
What do the different types of controls for immunofluorescence do?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsPrimary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on March 11, 2024
What is a control group?
Antibodies and Proteomics BioconjugationAntibody and Protein Labelingimmunofluorescence


Posted on March 11, 2024
What are the different types of control groups?
Antibodies and Proteomics BioconjugationAntibody and Protein Labelingimmunofluorescence


Posted on March 11, 2024
What is a negative control in an experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsimmunofluorescence


Posted on March 11, 2024
What is an example of a negative control in an experiment?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsimmunofluorescence


Posted on January 16, 2024
How do I label an animal cell?
Antibodies and ProteomicsMicroscopyfluorescence microscopeimmunofluorescence


Posted on December 7, 2023
What are the advantages of tagging fluorescent proteins (FPs) to label target proteins?
Antibodies and ProteomicsAntibody and Protein LabelingGreen Fluorescenceimmunofluorescence


Posted on December 7, 2023
What are the disadvantages of tagging fluorescent proteins (FPs) to label target proteins?
Antibodies and ProteomicsAntibody and Protein LabelingGreen Fluorescenceimmunofluorescence


Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence


Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on May 9, 2020
What are natural killer cells?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)fluorescence microscopeimmunofluorescence


Posted on May 9, 2020
What is a buffy coat?
DNA extractionIHCImmunohistochemistry (IHC)centrifugefluorescence microscopeimmunofluorescence


Posted on May 9, 2020
Which markers can be used to differentiate between human monocytes and macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on April 24, 2020
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence


Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 17, 2020
What would be the best marker in IHC/IF for bronchial epithelial cells?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescence


Posted on April 17, 2020
Is Gelatin or BSA best for blocking in immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAimmunofluorescence Chemical Reagents


Posted on April 17, 2020
What is the best blocking buffer for immunofluorescence ?
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence


Posted on April 17, 2020
What are the advantages and disadvantages of varying incubation time and temperature for immunofluorescence staining of tissue?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescenceIHC


Posted on February 25, 2020
What is the difference between immunofluorescence, immunohistochemistry and immunocytochemistry?
immunocytochemistryimmunohistochemistryimmunofluorescence


Posted on September 16, 2019
Why are cells washed?
bufferfluorescence imagingimmunofluorescencelive cell analysisno washcell cultureisoelectric point isotonic