logo
AAT Bioquest

FAQ

Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence


Posted on June 1, 2020
Why streptavidin is better than avidin for binding biotin?
Antibodies and Proteomics Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)BiotinBiotin and StreptavidinImmunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on May 9, 2020
Which markers can be used to differentiate between human monocytes and macrophages?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)flow cytometryimmunofluorescence


Posted on May 9, 2020
What is a buffy coat?
DNA extractionIHCImmunohistochemistry (IHC)centrifugefluorescence microscopeimmunofluorescence


Posted on May 9, 2020
What are natural killer cells?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)fluorescence microscopeimmunofluorescence


Posted on April 24, 2020
How can you detect endogenous expression of early endosome and lysosome in immunofluorescence?
Chemical ReagentsBSABuffer Preparations and RecipesImmunohistochemistry (IHC)LysosomesPBSbufferimmunofluorescence


Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence


Posted on April 17, 2020
What are the advantages and disadvantages of varying incubation time and temperature for immunofluorescence staining of tissue?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescenceIHC


Posted on April 17, 2020
What is the best blocking buffer for immunofluorescence ?
Antibody and Protein LabelingBSABuffer Preparations and RecipesImmunohistochemistry (IHC)PBSbufferimmunofluorescence


Posted on April 17, 2020
Is Gelatin or BSA best for blocking in immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingBSAimmunofluorescence Chemical Reagents


Posted on April 17, 2020
What would be the best marker in IHC/IF for bronchial epithelial cells?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)immunofluorescence


Posted on February 25, 2020
What is the difference between immunofluorescence, immunohistochemistry and immunocytochemistry?
immunocytochemistryimmunohistochemistryimmunofluorescence


Posted on September 16, 2019
Why are cells washed?
bufferfluorescence imagingimmunofluorescencelive cell analysisno washcell cultureisoelectric point isotonic