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TF1-dUTP *1 mM in Tris Buffer (pH 7.5)*

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Physical properties
Molecular weight738.43
SolventWater
Spectral properties
Correction Factor (280 nm)0.187
Extinction coefficient (cm -1 M -1)20000
Excitation (nm)341
Emission (nm)448
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Molecular weight
738.43
Correction Factor (280 nm)
0.187
Extinction coefficient (cm -1 M -1)
20000
Excitation (nm)
341
Emission (nm)
448
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF1-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set. (Spectrum Aqua™ is the trademark of Vysis).

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of TF1-dUTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM135.422 µL677.112 µL1.354 mL6.771 mL13.542 mL
5 mM27.084 µL135.422 µL270.845 µL1.354 mL2.708 mL
10 mM13.542 µL67.711 µL135.422 µL677.112 µL1.354 mL

Molarity calculator

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Spectrum


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Spectral properties

Correction Factor (280 nm)0.187
Extinction coefficient (cm -1 M -1)20000
Excitation (nm)341
Emission (nm)448

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
TF2-dUTP *1 mM in Tris Buffer (pH 7.5)*503525750000.09
TF3-dUTP *1 mM in Tris Buffer (pH 7.5)*5545787500010.179
TF4-dUTP *1 mM in Tris Buffer (pH 7.5)*578602900000.436
TF5-dUTP *1 mM in Tris Buffer (pH 7.5)*6496642500000.027
Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*552578900000.178
DEAC-dUTP *1 mM in Tris Buffer (pH 7.5)*411472-0.12

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References


View all 9 references: Citation Explorer
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953