TF1-dUTP *1 mM in TE Buffer (pH 7.5)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 931.56 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 35000 |
Excitation (nm) | 420 |
Emission (nm) | 461 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Fluorescent in situ hybridization (FISH), Nucleic Acid Building Blocks, RNA Purification & Analysis
Molecular weight 931.56 | Extinction coefficient (cm -1 M -1) 35000 | Excitation (nm) 420 | Emission (nm) 461 |
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TF1-dUTP conjugate can be used as a blue fluorescence color with Spectrum Aqua™ filter set. (Spectrum Aqua™ is the trademark of Vysis).
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of TF1-dUTP *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 107.347 µL | 536.734 µL | 1.073 mL | 5.367 mL | 10.735 mL |
5 mM | 21.469 µL | 107.347 µL | 214.694 µL | 1.073 mL | 2.147 mL |
10 mM | 10.735 µL | 53.673 µL | 107.347 µL | 536.734 µL | 1.073 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 35000 |
Excitation (nm) | 420 |
Emission (nm) | 461 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
TF2-dUTP *1 mM in TE Buffer (pH 7.5)* | 503 | 525 | 75000 | 0.09 |
TF3-dUTP *1 mM in TE Buffer (pH 7.5)* | 554 | 578 | 750001 | 0.179 |
TF4-dUTP *1 mM in TE Buffer (pH 7.5)* | 578 | 602 | 90000 | 0.436 |
TF5-dUTP *1 mM in TE Buffer (pH 7.5)* | 649 | 664 | 250000 | 0.027 |
Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)* | 552 | 578 | 90000 | 0.178 |
DEAC-dUTP *1 mM in TE Buffer (pH 7.5)* | 411 | 472 | - | 0.12 |
References
View all 9 references: Citation Explorer
A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405
Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518
Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229
A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145
Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166
Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953
Application notes
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