bBBr [Dibromobimane] *CAS 68654-25-1*
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Physical properties
Molecular weight | 350.01 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 394 |
Emission (nm) | 490 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
CAS 68654-25-1 | Molecular weight 350.01 | Excitation (nm) 394 | Emission (nm) 490 |
dBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. Dibromobimane is a unique fluorogenic crosslinking reagent for proteins because it is unlikely to fluoresce until both of its alkylating groups have reacted. It has been used to crosslink thiols in myosin, hemoglobin, Escherichia coli lactose permease and mitochondrial ATPase. Dibromobimane is also used to probe for the proximity of dual-cysteine mutagenesis sites in ArsA ATPase and to crosslink thiols in actin, myosin and P-glycoprotein.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of bBBr [Dibromobimane] *CAS 68654-25-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 285.706 µL | 1.429 mL | 2.857 mL | 14.285 mL | 28.571 mL |
5 mM | 57.141 µL | 285.706 µL | 571.412 µL | 2.857 mL | 5.714 mL |
10 mM | 28.571 µL | 142.853 µL | 285.706 µL | 1.429 mL | 2.857 mL |
Molarity calculator
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References
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Journal: AIDS Res Hum Retroviruses (2000): 1929
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Journal: Am J Physiol (1999): L191
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Journal: Free Radic Biol Med (1998): 803
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Journal: Clin Chem (1998): 825
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Journal: Clin Chem (1998): 825
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Interaction of glucose and metformin with isolated red cell membrane
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Journal: Arzneimittelforschung (1996): 773
Bromobimane probes for thiols
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Journal: Methods Enzymol (1995): 133
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Journal: Methods Enzymol (1995): 133