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Bodi Fluor™ 488 amine

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Physical properties
Molecular weight462.23
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
462.23
Bodi Fluor 488 amine is an excellent building block for developing fluorescent probes. Bodi Fluor 488 is the same fluorophore as BODIPY FL (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene FL). It is a popular lipophilic fluorescent dye molecule used in biology, chemistry, and materials science. BODI Fluor 488 has high fluorescence quantum yields, photostability, and brightness. When excited with light in the blue to green range (around 488 to 515 nanometers), it emits around 510 to 520 nanometers. Researchers and scientists use BODIPY FL for various purposes, such as labeling biomolecules (proteins, nucleic acids, etc.) for fluorescence microscopy, flow cytometry, and other imaging techniques. It can also be employed as a probe for sensing and detecting various analytes or as a fluorescent marker in chemical and biological assays.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM216.343 µL1.082 mL2.163 mL10.817 mL21.634 mL
5 mM43.269 µL216.343 µL432.685 µL2.163 mL4.327 mL
10 mM21.634 µL108.171 µL216.343 µL1.082 mL2.163 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3*504510810000.0150.018
Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2*504510810000.0150.018

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References


View all 50 references: Citation Explorer
Engineering Metastability into a Virus-like Particle to Enable Triggered Dissociation.
Authors: Starr, Caleb A and Nair, Smita and Huang, Sheng-Yuan and Hagan, Michael F and Jacobson, Stephen C and Zlotnick, Adam
Journal: Journal of the American Chemical Society (2023): 2322-2331
Pore-like diffusion barriers in murine cardiac myocytes.
Authors: Deisl, Christine and Chung, Jay A and Hilgemann, Donald W
Journal: bioRxiv : the preprint server for biology (2023)
Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 Activity in the Mammalian Polyamine Transport System.
Authors: Houdou, Marine and Jacobs, Nathalie and Coene, Jonathan and Azfar, Mujahid and Vanhoutte, Roeland and Van den Haute, Chris and Eggermont, Jan and Daniëls, Veronique and Verhelst, Steven H L and Vangheluwe, Peter
Journal: Biomolecules (2023)
High sensitivity (zeptomole) detection of BODIPY-labelled heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts.
Authors: Maciej-Hulme, Marissa L and Leprince, Anaëlle C N and Lavin, Andre and Guimond, Scott E and Turnbull, Jeremy E and Pelletier, Julien and Yates, Edwin A and Powell, Andrew K and Skidmore, Mark A
Journal: Analytical methods : advancing methods and applications (2023): 1461-1469
Resistin Modulates Low-Density Lipoprotein Cholesterol Uptake in Human Placental Explants via PCSK9.
Authors: Nava-Salazar, Sonia and Flores-Pliego, Arturo and Pérez-Martínez, Giovanni and Parra-Hernández, Sandra and Vanoye-Carlo, America and Ibarguengoitia-Ochoa, Francisco and Perichart-Perera, Otilia and Reyes-Muñoz, Enrique and Solis-Paredes, Juan Mario and Espino Y Sosa, Salvador and Estrada-Gutierrez, Guadalupe
Journal: Reproductive sciences (Thousand Oaks, Calif.) (2022): 3242-3253
Design, Spectral Characteristics, Photostability, and Possibilities for Practical Application of BODIPY FL-Labeled Thioterpenoid.
Authors: Guseva, Galina B and Antina, Elena V and Berezin, Mikhail B and Smirnova, Anastassia S and Pavelyev, Roman S and Gilfanov, Ilmir R and Shevchenko, Oksana G and Pestova, Svetlana V and Izmest'ev, Evgeny S and Rubtsova, Svetlana A and Ostolopovskaya, Olga V and Efimov, Sergey V and Klochkov, Vladimir V and Rakhmatullin, Ilfat Z and Timerova, Ayzira F and Khodov, Ilya A and Lodochnikova, Olga A and Islamov, Daut R and Dorovatovskii, Pavel V and Nikitina, Liliya E and Boichuk, Sergei V
Journal: Bioengineering (Basel, Switzerland) (2022)
Microscopy-Based Assessment of Fatty Acid Uptake and Lipid Accumulation in Cultured Cells.
Authors: Khan, Danish and Ara, Tarannum and Munjal, Aastha and Mishra, Sneha and Sundaresan, Nagalingam Ravi
Journal: Current protocols (2022): e626
Robust Chemical Strategy for Stably Labeling Polyester-Based Nanoparticles with BODIPY Fluorophores.
Authors: Alferiev, Ivan S and Fishbein, Ilia and Levy, Robert J and Chorny, Michael
Journal: ACS applied polymer materials (2022): 1196-1206
A Spectroscopic Technique to Simultaneously Characterize Fatty Acid Uptake, Mitochondrial Activity, Vascularity, and Oxygen Saturation for Longitudinal Studies In Vivo.
Authors: Deutsch, Riley J and D'Agostino, Victoria W and Sunassee, Enakshi D and Kwan, Michelle and Madonna, Megan C and Palmer, Gregory and Crouch, Brian T and Ramanujam, Nimmi
Journal: Metabolites (2022)
Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells.
Authors: Fan, Wei and Li, Xiaoling
Journal: Bio-protocol (2022)