Bodi Fluor™ 488 amine
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 462.23 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Molecular weight 462.23 |
Bodi Fluor 488 amine is an excellent building block for developing fluorescent probes. Bodi Fluor 488 is the same fluorophore as BODIPY FL (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene FL). It is a popular lipophilic fluorescent dye molecule used in biology, chemistry, and materials science. BODI Fluor 488 has high fluorescence quantum yields, photostability, and brightness. When excited with light in the blue to green range (around 488 to 515 nanometers), it emits around 510 to 520 nanometers. Researchers and scientists use BODIPY FL for various purposes, such as labeling biomolecules (proteins, nucleic acids, etc.) for fluorescence microscopy, flow cytometry, and other imaging techniques. It can also be employed as a probe for sensing and detecting various analytes or as a fluorescent marker in chemical and biological assays.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Bodi Fluor™ 488 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 216.343 µL | 1.082 mL | 2.163 mL | 10.817 mL | 21.634 mL |
5 mM | 43.269 µL | 216.343 µL | 432.685 µL | 2.163 mL | 4.327 mL |
10 mM | 21.634 µL | 108.171 µL | 216.343 µL | 1.082 mL | 2.163 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Bodi Fluor™ 488 acid [equivalent to Bodipy® FL] *CAS 165599-63-3* | 504 | 510 | 81000 | 0.015 | 0.018 |
Bodi Fluor™ 488 SE [equivalent to Bodipy® FL, SE] *CAS 146616-66-2* | 504 | 510 | 81000 | 0.015 | 0.018 |
Images
Figure 1. Bodi Fluor 488 amine is an excellent building block for developing fluorescent probes. Bodi Fluor 488 is the same fluorophore as BODIPY FL (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene FL). It is a popular lipophilic fluorescent dye molecule used in biology, chemistry, and materials science.
References
View all 50 references: Citation Explorer
Engineering Metastability into a Virus-like Particle to Enable Triggered Dissociation.
Authors: Starr, Caleb A and Nair, Smita and Huang, Sheng-Yuan and Hagan, Michael F and Jacobson, Stephen C and Zlotnick, Adam
Journal: Journal of the American Chemical Society (2023): 2322-2331
Authors: Starr, Caleb A and Nair, Smita and Huang, Sheng-Yuan and Hagan, Michael F and Jacobson, Stephen C and Zlotnick, Adam
Journal: Journal of the American Chemical Society (2023): 2322-2331
Pore-like diffusion barriers in murine cardiac myocytes.
Authors: Deisl, Christine and Chung, Jay A and Hilgemann, Donald W
Journal: bioRxiv : the preprint server for biology (2023)
Authors: Deisl, Christine and Chung, Jay A and Hilgemann, Donald W
Journal: bioRxiv : the preprint server for biology (2023)
Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 Activity in the Mammalian Polyamine Transport System.
Authors: Houdou, Marine and Jacobs, Nathalie and Coene, Jonathan and Azfar, Mujahid and Vanhoutte, Roeland and Van den Haute, Chris and Eggermont, Jan and Daniëls, Veronique and Verhelst, Steven H L and Vangheluwe, Peter
Journal: Biomolecules (2023)
Authors: Houdou, Marine and Jacobs, Nathalie and Coene, Jonathan and Azfar, Mujahid and Vanhoutte, Roeland and Van den Haute, Chris and Eggermont, Jan and Daniëls, Veronique and Verhelst, Steven H L and Vangheluwe, Peter
Journal: Biomolecules (2023)
High sensitivity (zeptomole) detection of BODIPY-labelled heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts.
Authors: Maciej-Hulme, Marissa L and Leprince, Anaëlle C N and Lavin, Andre and Guimond, Scott E and Turnbull, Jeremy E and Pelletier, Julien and Yates, Edwin A and Powell, Andrew K and Skidmore, Mark A
Journal: Analytical methods : advancing methods and applications (2023): 1461-1469
Authors: Maciej-Hulme, Marissa L and Leprince, Anaëlle C N and Lavin, Andre and Guimond, Scott E and Turnbull, Jeremy E and Pelletier, Julien and Yates, Edwin A and Powell, Andrew K and Skidmore, Mark A
Journal: Analytical methods : advancing methods and applications (2023): 1461-1469
Resistin Modulates Low-Density Lipoprotein Cholesterol Uptake in Human Placental Explants via PCSK9.
Authors: Nava-Salazar, Sonia and Flores-Pliego, Arturo and Pérez-Martínez, Giovanni and Parra-Hernández, Sandra and Vanoye-Carlo, America and Ibarguengoitia-Ochoa, Francisco and Perichart-Perera, Otilia and Reyes-Muñoz, Enrique and Solis-Paredes, Juan Mario and Espino Y Sosa, Salvador and Estrada-Gutierrez, Guadalupe
Journal: Reproductive sciences (Thousand Oaks, Calif.) (2022): 3242-3253
Authors: Nava-Salazar, Sonia and Flores-Pliego, Arturo and Pérez-Martínez, Giovanni and Parra-Hernández, Sandra and Vanoye-Carlo, America and Ibarguengoitia-Ochoa, Francisco and Perichart-Perera, Otilia and Reyes-Muñoz, Enrique and Solis-Paredes, Juan Mario and Espino Y Sosa, Salvador and Estrada-Gutierrez, Guadalupe
Journal: Reproductive sciences (Thousand Oaks, Calif.) (2022): 3242-3253
Design, Spectral Characteristics, Photostability, and Possibilities for Practical Application of BODIPY FL-Labeled Thioterpenoid.
Authors: Guseva, Galina B and Antina, Elena V and Berezin, Mikhail B and Smirnova, Anastassia S and Pavelyev, Roman S and Gilfanov, Ilmir R and Shevchenko, Oksana G and Pestova, Svetlana V and Izmest'ev, Evgeny S and Rubtsova, Svetlana A and Ostolopovskaya, Olga V and Efimov, Sergey V and Klochkov, Vladimir V and Rakhmatullin, Ilfat Z and Timerova, Ayzira F and Khodov, Ilya A and Lodochnikova, Olga A and Islamov, Daut R and Dorovatovskii, Pavel V and Nikitina, Liliya E and Boichuk, Sergei V
Journal: Bioengineering (Basel, Switzerland) (2022)
Authors: Guseva, Galina B and Antina, Elena V and Berezin, Mikhail B and Smirnova, Anastassia S and Pavelyev, Roman S and Gilfanov, Ilmir R and Shevchenko, Oksana G and Pestova, Svetlana V and Izmest'ev, Evgeny S and Rubtsova, Svetlana A and Ostolopovskaya, Olga V and Efimov, Sergey V and Klochkov, Vladimir V and Rakhmatullin, Ilfat Z and Timerova, Ayzira F and Khodov, Ilya A and Lodochnikova, Olga A and Islamov, Daut R and Dorovatovskii, Pavel V and Nikitina, Liliya E and Boichuk, Sergei V
Journal: Bioengineering (Basel, Switzerland) (2022)
Microscopy-Based Assessment of Fatty Acid Uptake and Lipid Accumulation in Cultured Cells.
Authors: Khan, Danish and Ara, Tarannum and Munjal, Aastha and Mishra, Sneha and Sundaresan, Nagalingam Ravi
Journal: Current protocols (2022): e626
Authors: Khan, Danish and Ara, Tarannum and Munjal, Aastha and Mishra, Sneha and Sundaresan, Nagalingam Ravi
Journal: Current protocols (2022): e626
Robust Chemical Strategy for Stably Labeling Polyester-Based Nanoparticles with BODIPY Fluorophores.
Authors: Alferiev, Ivan S and Fishbein, Ilia and Levy, Robert J and Chorny, Michael
Journal: ACS applied polymer materials (2022): 1196-1206
Authors: Alferiev, Ivan S and Fishbein, Ilia and Levy, Robert J and Chorny, Michael
Journal: ACS applied polymer materials (2022): 1196-1206
A Spectroscopic Technique to Simultaneously Characterize Fatty Acid Uptake, Mitochondrial Activity, Vascularity, and Oxygen Saturation for Longitudinal Studies In Vivo.
Authors: Deutsch, Riley J and D'Agostino, Victoria W and Sunassee, Enakshi D and Kwan, Michelle and Madonna, Megan C and Palmer, Gregory and Crouch, Brian T and Ramanujam, Nimmi
Journal: Metabolites (2022)
Authors: Deutsch, Riley J and D'Agostino, Victoria W and Sunassee, Enakshi D and Kwan, Michelle and Madonna, Megan C and Palmer, Gregory and Crouch, Brian T and Ramanujam, Nimmi
Journal: Metabolites (2022)
Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells.
Authors: Fan, Wei and Li, Xiaoling
Journal: Bio-protocol (2022)
Authors: Fan, Wei and Li, Xiaoling
Journal: Bio-protocol (2022)