Cy5 tyramide

For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. Cy5 tyramide contains the bright Cy5 that can be readily detected with the standard Cy5 filter set.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11066	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5 tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	112.36 µL	561.798 µL	1.124 mL	5.618 mL	11.236 mL
5 mM	22.472 µL	112.36 µL	224.719 µL	1.124 mL	2.247 mL
10 mM	11.236 µL	56.18 µL	112.36 µL	561.798 µL	1.124 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	890 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Arctigenin Prevents Metabolic Dysfunction-associated Steatohepatitis by Inhibiting NLRP3/GSDMD-N Axis in Macrophages

Authors:

Xue, Rui and Jiang, Lu and Zhang, Qian-Ren and Wang, Qing-Jing and Yang, Rui-Xu and Ren, Tian-Yi and Pan, Qin and Fan, Jian-Gao

Journal:

Journal of Clinical and Translational Hepatology (2025): 0--0

Immunostimulatory DNA Tetrahedron-Based Nanovaccine Combined With Immune Checkpoint PD-1 Blockade for Boosting Systemic Immune Responses Against Oral Squamous Cell Carcinoma

Authors:

Yang, Xueting and Wang, Yun and Yang, Yan and Zhang, Sicheng and Wang, Dianri and Luo, Yi and Shui, Chunyan and Cai, Yongcong and Yang, Ruoyi and Dong, Shuang and others,

Journal:

Aggregate (2025): e70061

Anti-TGF-$\beta$/PD-L1 bispecific antibody synergizes with radiotherapy to enhance antitumor immunity and mitigate radiation-induced pulmonary fibrosis

Authors:

Wu, Yuze and Yan, Yuheng and Guo, Yarong and Niu, Mengke and Zhou, Binghan and Zhang, Jing and Zhou, Pengfei and Chu, Qian and Mei, Qi and Yi, Ming and others,

Journal:

Journal of Hematology \& Oncology (2025): 1--20

Anti-inflammatory effects of MerTK by inducing M2 macrophage polarization via PI3K/Akt/GSK-3$\beta$ pathway in gout

Authors:

Chen, Fangfang and Li, Yixuan and Zhao, Li and Lin, Cong and Zhou, Yingzi and Ye, Wenjing and Wan, Weiguo and Zou, Hejian and Xue, Yu

Journal:

International Immunopharmacology (2024): 112942

Contribution of Tregs to the promotion of constructive remodeling after decellularized extracellular matrix material implantation

Authors:

Jiang, Hongjing and Sun, Xuheng and Wu, Yindi and Xu, Jianyi and Xiao, Cong and Liu, Qing and Fang, Lijun and Liang, Yuanfeng and Zhou, Jiahui and Wu, Yueheng and others,

Journal:

Materials Today Bio (2024): 101151

References

View all 74 references: Citation Explorer

Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification

Authors:

Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.

Journal:

J Vet Diagn Invest (2015): 326

Tyramide Signal Amplification for Immunofluorescent Enhancement

Authors:

Faget L, Hnasko TS.

Journal:

Methods Mol Biol (2015): 161

Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification

Authors:

Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.

Journal:

J Food Prot (2014): 100

Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis

Authors:

Stack EC, Wang C, Roman KA, Hoyt CC.

Journal:

Methods (2014): 46

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells

Authors:

Garrigues HJ, Rubinchikova YE, Rose TM.

Journal:

Virology (2014): 75

Physical properties

Molecular weight	890.00
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 10, 2025