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Cy7 tetrazine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1162.54 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.036 |
| Correction Factor (482 nm) | 0.0005 |
| Correction Factor (565 nm) | 0.0193 |
| Correction Factor (650 nm) | 0.165 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 756 |
| Emission (nm) | 779 |
| Quantum yield | 0.3 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 1162.54 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.036 | Correction Factor (482 nm) 0.0005 | Correction Factor (565 nm) 0.0193 | Correction Factor (650 nm) 0.165 | Extinction coefficient (cm -1 M -1) 250000 | Excitation (nm) 756 | Emission (nm) 779 | Quantum yield 0.3 |
Cy7 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules (e.g., peptides, proteins and oligos etc) for NIR fluorescence in vivo imaging and other fluorescence-based biological applications. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to drug discovery, life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy7 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 86.019 µL | 430.093 µL | 860.185 µL | 4.301 mL | 8.602 mL |
| 5 mM | 17.204 µL | 86.019 µL | 172.037 µL | 860.185 µL | 1.72 mL |
| 10 mM | 8.602 µL | 43.009 µL | 86.019 µL | 430.093 µL | 860.185 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.05 |
| Correction Factor (280 nm) | 0.036 |
| Correction Factor (482 nm) | 0.0005 |
| Correction Factor (565 nm) | 0.0193 |
| Correction Factor (650 nm) | 0.165 |
| Extinction coefficient (cm -1 M -1) | 250000 |
| Excitation (nm) | 756 |
| Emission (nm) | 779 |
| Quantum yield | 0.3 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Cy7 tyramide | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
| Cy7 Styramide *Superior Replacement for Cy7 tyramide* | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
| Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| Cy5 tetrazine | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| Cy3B Tetrazine | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
| PerCP-Cy7 | 757 | 783 | 350000 | - | - | - |
Images
Figure 1. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction. Cy7 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules (e.g., peptides, proteins and oligos etc) for NIR fluorescence in vivo imaging and other fluorescence-based biological applications.
References
View all 3 references: Citation Explorer
Signal Amplification Pretargeted PET/Fluorescence Imaging Based on Human Serum Albumin-Encapsulated GdF3 Nanoparticles for Diagnosis of Ovarian Cancer.
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964
Enabling In Vivo Photocatalytic Activation of Rapid Bioorthogonal Chemistry by Repurposing Silicon-Rhodamine Fluorophores as Cytocompatible Far-Red Photocatalysts.
Authors: Wang, Chuanqi and Zhang, He and Zhang, Tao and Zou, Xiaoyu and Wang, Hui and Rosenberger, Julia E and Vannam, Raghu and Trout, William S and Grimm, Jonathan B and Lavis, Luke D and Thorpe, Colin and Jia, Xinqiao and Li, Zibo and Fox, Joseph M
Journal: Journal of the American Chemical Society (2021): 10793-10803
Authors: Wang, Chuanqi and Zhang, He and Zhang, Tao and Zou, Xiaoyu and Wang, Hui and Rosenberger, Julia E and Vannam, Raghu and Trout, William S and Grimm, Jonathan B and Lavis, Luke D and Thorpe, Colin and Jia, Xinqiao and Li, Zibo and Fox, Joseph M
Journal: Journal of the American Chemical Society (2021): 10793-10803
NIR-Cyanine Dye Linker: a Promising Candidate for Isochronic Fluorescence Imaging in Molecular Cancer Diagnostics and Therapy Monitoring.
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41