Gelite™ Orange Nucleic Acid Gel Staining Kit

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

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H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
UNSPSC	41116134

Alternative formats

Gelite™ Green Nucleic Acid Gel Staining Kit

Overview SDS Protocol

Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. As with Helixyte™ Green stain, this remarkable sensitivity can be attributed to a combination of unique dye characteristics. Because the nucleic acid-bound Gelite™ Orange dye exhibits excitation maxima at both ~495 nm and ~300 nm (the emission maximum is ~537 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp- and argon-ion laser-based gel scanners. Our Gelite™ Orange Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Orange nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.

Platform

Transilluminator

Excitation	254 nm or 300 nm
Emission	Long path green filter (ex. SYBR or GelStar)

Components

Example protocol

PREPARATION OF WORKING SOLUTION

Add 1 μL of Gelite™ Orange (Component A) into 200 μL of 5X Gel Loading Buffer (Component B). Protect the working solution from light by covering it with foil or placing it in the dark.

SAMPLE EXPERIMENTAL PROTOCOL

Prepare DNA samples as you desired.
Add 4 µL of Gelite™ Orange working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
Run gels based on your standard protocol.
Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

Images

Figure 1. 160 ng of 1 kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Orange and SYBR® Gold, and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

References

View all 79 references: Citation Explorer

SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups
Authors: Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.
Journal: PLoS One (2014): e89963

Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species
Authors: Anthony Johnson AM, Dasgupta I, Sai Gopal DV.
Journal: J Virol Methods (2014): 9

Development of both multiple PCR and real-time SYBR green PCR for the detection of Vibrio cholerae non-O1/O139 serogroups
Authors: Li F, Kan B, Wang D.
Journal: Zhonghua Liu Xing Bing Xue Za Zhi (2014): 66

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR
Authors: Siljo A, Bhat AI, Biju CN.
Journal: Virusdisease (2014): 137

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85

Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72

Primer evaluation and adaption for cost-efficient SYBR Green-based qPCR and its applicability for specific quantification of methanogens
Authors: Reitschuler C, Lins P, Illmer P.
Journal: World J Microbiol Biotechnol (2014): 293

Development and comparative evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of West Nile virus in human patients
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)

Multiplex SYBR(R) green-real time PCR (qPCR) assay for the detection and differentiation of Bartonella henselae and Bartonella clarridgeiae in cats
Authors: Staggemeier R, Pilger DA, Spilki FR, Cantarelli VV.
Journal: Rev Inst Med Trop Sao Paulo (2014): 93

Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101