iFluor® 555 Tyramide

iFluor® 555 Tyramide is an orange-fluorescent signal amplification reagent for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Tyramide Signal Amplification: Enhances detection sensitivity through HRP-catalyzed fluorescence amplification.
Spectral Properties: Excitation at 557 nm and emission at 570 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of fluorescent labels near target sites.
Filter Compatibility: Supports fluorescence imaging using standard Cy3/TRITC filter sets.
Enhanced Fluorescence: Produces stronger signals than Alexa Fluor® 555 Tyramide under manufacturer-tested conditions.

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using iFluor® 555 tyramide (Cat No. 45105) and detected with a TRITC/Cy3 filter set. Nuclei (blue) were counterstained with DAPI (Cat No. 17507).

Protocol

SDS

Catalog	Size	Price	Quantity
45105	200 Slides	Price

Overview

For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 555 tyramide contains the bright iFluor® 555 that can be readily detected with the standard Cy3 filter set. It is an excellent replacement for Alexa Fluor® 555 tyramide (Alexa Fluor® is the trademark of ThermoFisher), Cy3 tyramide, or other comparable fluorescent tyramide conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 555 Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.565 µL	477.824 µL	955.648 µL	4.778 mL	9.556 mL
5 mM	19.113 µL	95.565 µL	191.13 µL	955.648 µL	1.911 mL
10 mM	9.556 µL	47.782 µL	95.565 µL	477.824 µL	955.648 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1046.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Structural mechanism of biased activation of T cell receptor by a high-affinity antibody

Authors:

Li, Xiang and Huang, Buliao and Shao, Enze and Zhu, Yuwei and Yang, Dehui and Chen, Yan and Gao, Wenbo and Lu, Zebin and Lu, Fengliang and Zhang, Anqi and others

Journal:

Vita (2026)

CD46 and DSG2 synergistically mediate human adenovirus type 7 infection

Authors:

Ye, Lihua and Mo, Chuncong and Yuan, Jinwei and Su, Yalin and Zhou, Chengxing and Yang, Yujie and Li, Xiao and Liu, Wenkuan and Zeng, Rongjie and Zhou, Rong and others

Journal:

Journal of Virology (2026): e00136--26

Omics insights into MYBL2-promoted cancer stem-like cells driving ferroptosis resistance in hepatocellular carcinoma

Authors:

Chen, Yuting and Li, Xu and Luo, Chaochao and Lin, Guanchuan and Zhou, Xuewen and Wen, Zebin and Su, Huanhou and Meng, Wei and Chen, Caiming and Lu, Jiameng and others

Journal:

Journal of Advanced Research (2026)

Metformin Ameliorates Early Brain Injury After Subarachnoid Hemorrhage Via Improving Endoplasmic Reticulum Stress and Mitochondrial Stress-Mediated Ca2+ Imbalance

Authors:

Zhang, Zhonghua and Zhang, Jingbei and Li, Liping and Zong, Lin

Journal:

Molecular Neurobiology (2026): 256

MEIS1-mediated Apoptosis via TNFR1 in Endometriosis

Authors:

Wang, Wenwen and Fu, Fangfang and Li, Yan and Li, Sha and Yuan, Ming and Wang, Tian and Ren, Wu and Wei, Jia and Chen, Dan and Wang, Shixuan and others

Journal:

Reproductive Sciences (2025): 1--12

Physical properties

Molecular weight	1046.41
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 8, 2026