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Live or Dead™ Fixable Dead Cell Staining Kit *IR Fluorescence*
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
| Excitation (nm) | 853 |
| Emission (nm) | 877 |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Excitation (nm) 853 | Emission (nm) 877 |
Platform
Flow cytometer
| Excitation | 808 nm laser |
| Emission | 885/40 or IR Channel |
Components
Example protocol
AT A GLANCE
Prepare samples in HHBS buffer (0.5 mL/assay).
Wash cells and replace with fresh HHBS buffer.
Add Stain It™ IR fluorescence to the cell suspension.
- Stain the cells at room temperature or 37°C for 20 - 60 minutes
Wash the cells.
Fix the cells (optional).
Examine the sample with a flow cytometer using an 808 nm laser with an 885/40 nm or IR channel emission filter.
Before starting the experiment, thaw all the components at room temperature.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 200 µL of DMSO (Component B) to the vial of Stain It™ IR fluorescence (Component A) to make a 500X Stain It™ IR fluorescence stock solution.
SAMPLE EXPERIMENTAL PROTOCOL
Prepare cells using 1X Hanks and 20 mM Hepes buffer (HHBS) or a sodium azide-free and serum/protein-free buffer of your choice.
Wash the cells once with HHBS or the azide- and serum/protein-free buffer of your choice.
Resuspend the cells at 5 - 10 × 106/mL in HHBS or in the azide- and serum/protein-free buffer of your choice.
Add 1 µL of 500X Stain It™ IR fluorescence stock solution to 0.5 mL of cells/assay and mix well.
Incubate at room temperature or 37°C, 5% CO2 incubator for 20 - 60 minutes, protected from light.
Note: The optimal stain concentrations and incubation time should be experimentally determined for different cell lines.
Wash the cells twice and resuspend cells with HHBS or a buffer of your choice.
Fix cells as desired (optional).
Analyze cells with a flow cytometer using the appropriate excitation/emission specifications found in the 'Key Parameters' section.
