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OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 512.36 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.19 |
| Extinction coefficient (cm -1 M -1) | 85000 |
| Excitation (nm) | 513 |
| Emission (nm) | 533 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 512.36 | Correction Factor (280 nm) 0.19 | Extinction coefficient (cm -1 M -1) 85000 | Excitation (nm) 513 | Emission (nm) 533 |
OG514 is the same fluorophore as Oregon Green 514 (Oregon Green® is the trademark of ThermoFisher). OG514 acid is an amine-reactive compound used for creating green, fluorescent bioconjugates. It has excitation/emission maxima approximately 489/526 nm. It is an excellent replacement for rhodamine 6G that has poor solubility in most common solvents, thus practically hard to use. Although it is a fluorescein derivative, it has significantly improved photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of OG514 acid [equivalent to Oregon Green® 514 carboxylic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 195.175 µL | 975.876 µL | 1.952 mL | 9.759 mL | 19.518 mL |
| 5 mM | 39.035 µL | 195.175 µL | 390.351 µL | 1.952 mL | 3.904 mL |
| 10 mM | 19.518 µL | 97.588 µL | 195.175 µL | 975.876 µL | 1.952 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.19 |
| Extinction coefficient (cm -1 M -1) | 85000 |
| Excitation (nm) | 513 |
| Emission (nm) | 533 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) | Correction Factor (260 nm) |
| ICG acid | 789 | 813 | 230000 | - | 0.076 | - |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.151 | 0.073 | 0.07 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 | 0.02 |
| Cy7NS acid | 756 | 779 | 250000 | 0.3 | 0.036 | 0.05 |
| AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | - | 0.153 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* | 336 | 455 | 5900 | - | 0.107 | - |
| EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | - | 0.107 | - |
| DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | - | - | - | - | 0.516 | - |
| BCECF acid *CAS#: 85138-49-4* | 504 | 527 | 90000 | - | - | - |
Show More (12) | ||||||
Images
Figure 1. OG514 is the same fluorophore as Oregon Green 514 (Oregon Green® is the trademark of ThermoFisher). OG514 acid is an amine-reactive compound used for creating green, fluorescent bioconjugates.
References
View all 13 references: Citation Explorer
Internalization and Subcellular Trafficking of Poly-l-lysine Dendrimers Are Impacted by the Site of Fluorophore Conjugation.
Authors: Avaritt, Brittany R and Swaan, Peter W
Journal: Molecular pharmaceutics (2015): 1961-9
Authors: Avaritt, Brittany R and Swaan, Peter W
Journal: Molecular pharmaceutics (2015): 1961-9
F-actin distribution and function during sexual development in Eimeria maxima.
Authors: Frölich, Sonja and Wallach, Michael
Journal: Parasitology (2015): 855-64
Authors: Frölich, Sonja and Wallach, Michael
Journal: Parasitology (2015): 855-64
[A fluorimetric microplate assay for detecting diarrheic shellfish poisoning toxins].
Authors: Huang, Haiyan and Huang, Aijun and Fu, Yingbin and Huang, Wei and Yu, Xintian and Liu, Jianjun
Journal: Wei sheng yan jiu = Journal of hygiene research (2012): 501-3, 507
Authors: Huang, Haiyan and Huang, Aijun and Fu, Yingbin and Huang, Wei and Yu, Xintian and Liu, Jianjun
Journal: Wei sheng yan jiu = Journal of hygiene research (2012): 501-3, 507
Comparative cytotoxicity of gambierol versus other marine neurotoxins.
Authors: Cagide, E and Louzao, M C and Espiña, B and Ares, I R and Vieytes, M R and Sasaki, M and Fuwa, H and Tsukano, C and Konno, Y and Yotsu-Yamashita, M and Paquette, L A and Yasumoto, T and Botana, L M
Journal: Chemical research in toxicology (2011): 835-42
Authors: Cagide, E and Louzao, M C and Espiña, B and Ares, I R and Vieytes, M R and Sasaki, M and Fuwa, H and Tsukano, C and Konno, Y and Yotsu-Yamashita, M and Paquette, L A and Yasumoto, T and Botana, L M
Journal: Chemical research in toxicology (2011): 835-42
Disruption of the actin cytoskeleton induces fluorescent glucose accumulation on the rat hepatocytes Clone 9.
Authors: Espiña, Begoña and Louzao, M Carmen and Ares, Isabel R and Fonfria, Eva S and Vilariño, Natalia and Vieytes, Mercedes R and Botana, Luis M
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2011): 653-60
Authors: Espiña, Begoña and Louzao, M Carmen and Ares, Isabel R and Fonfria, Eva S and Vilariño, Natalia and Vieytes, Mercedes R and Botana, Luis M
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2011): 653-60
Induction of actin cytoskeleton rearrangement by methyl okadaate--comparison with okadaic acid.
Authors: Vilariño, Natalia and Ares, Isabel R and Cagide, Eva and Louzao, M Carmen and Vieytes, Mercedes R and Yasumoto, Takeshi and Botana, Luis M
Journal: The FEBS journal (2008): 926-34
Authors: Vilariño, Natalia and Ares, Isabel R and Cagide, Eva and Louzao, M Carmen and Vieytes, Mercedes R and Yasumoto, Takeshi and Botana, Luis M
Journal: The FEBS journal (2008): 926-34
Comparative study of guest charge-charge interactions within silica sol-gel.
Authors: Gilliland, James W and Yokoyama, Kazushige and Yip, Wai Tak
Journal: The journal of physical chemistry. B (2005): 4816-23
Authors: Gilliland, James W and Yokoyama, Kazushige and Yip, Wai Tak
Journal: The journal of physical chemistry. B (2005): 4816-23
Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions.
Authors: Rusinova, Elena and Tretyachenko-Ladokhina, Vira and Vele, Oana E and Senear, Donald F and Alexander Ross, J B
Journal: Analytical biochemistry (2002): 18-25
Authors: Rusinova, Elena and Tretyachenko-Ladokhina, Vira and Vele, Oana E and Senear, Donald F and Alexander Ross, J B
Journal: Analytical biochemistry (2002): 18-25
Novel immuno-FRET assay method for Bacillus spores and Escherichia coli O157:H7.
Authors: Bruno, J G and Ulvick, S J and Uzzell, G L and Tabb, J S and Valdes, E R and Batt, C A
Journal: Biochemical and biophysical research communications (2001): 875-80
Authors: Bruno, J G and Ulvick, S J and Uzzell, G L and Tabb, J S and Valdes, E R and Batt, C A
Journal: Biochemical and biophysical research communications (2001): 875-80
Leakage responses to L-NAME differ with the fluorescent dye used to label albumin.
Authors: Rumbaut, R E and Harris, N R and Sial, A J and Huxley, V H and Granger, D N
Journal: The American journal of physiology (1999): H333-9
Authors: Rumbaut, R E and Harris, N R and Sial, A J and Huxley, V H and Granger, D N
Journal: The American journal of physiology (1999): H333-9