PRO-TAO-3 [equivalent to TO-PRO-3]
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 671.42 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 102000 |
Excitation (nm) | 642 |
Emission (nm) | 657 |
Quantum yield | 0.111 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
CAS 157199-63-8 | Molecular weight 671.42 | Extinction coefficient (cm -1 M -1) 102000 | Excitation (nm) 642 | Emission (nm) 657 | Quantum yield 0.111 |
PRO-TAO-3 is the same molecule to TO-PRO®-3 stain. TO-PRO®-3 and the other monomeric cyanine stains have wide applicability due to their low background and bright fluorescence. It is a carbocyanine monomer nucleic acid stain that can be well excited by a red laser at 633 nm or 647 nm with far-red fluorescence similar to Cy®5 dyes. It is useful as a nuclear counterstain and dead cell indicator. It is selective for detecting dsDNA (double-stranded nucleic acids). The cell-impermeable PRO-TTO-3 is widely used for flow cytometry and fluorescence microscopy as a long-wavelength fluorescence well separated from green and red fluorophores in multicolor applications. TO-PRO® is the trademark of ThermoFisher Scientific.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of PRO-TAO-3 [equivalent to TO-PRO-3] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 148.938 µL | 744.69 µL | 1.489 mL | 7.447 mL | 14.894 mL |
5 mM | 29.788 µL | 148.938 µL | 297.876 µL | 1.489 mL | 2.979 mL |
10 mM | 14.894 µL | 74.469 µL | 148.938 µL | 744.69 µL | 1.489 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 102000 |
Excitation (nm) | 642 |
Emission (nm) | 657 |
Quantum yield | 0.111 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8* | 642 | 657 | 1020001 | 0.111 |
References
View all 50 references: Citation Explorer
Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.
Authors: Escoter-Torres, Laura and Greulich, Franziska and Quagliarini, Fabiana and Wierer, Michael and Uhlenhaut, Nina Henriette
Journal: Nucleic acids research (2020)
Authors: Escoter-Torres, Laura and Greulich, Franziska and Quagliarini, Fabiana and Wierer, Michael and Uhlenhaut, Nina Henriette
Journal: Nucleic acids research (2020)
Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction.
Authors: Clementi, Elena and Inglin, Larissa and Beebe, Erin and Gsell, Corina and Garajova, Zuzana and Markkanen, Enni
Journal: BMC biology (2020): 36
Authors: Clementi, Elena and Inglin, Larissa and Beebe, Erin and Gsell, Corina and Garajova, Zuzana and Markkanen, Enni
Journal: BMC biology (2020): 36
DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.
Authors: Kyono, Yasuhiro and Raj, Samhitha and Sifuentes, Christopher J and Buisine, Nicolas and Sachs, Laurent and Denver, Robert J
Journal: Developmental biology (2020): 180-196
Authors: Kyono, Yasuhiro and Raj, Samhitha and Sifuentes, Christopher J and Buisine, Nicolas and Sachs, Laurent and Denver, Robert J
Journal: Developmental biology (2020): 180-196
Mithramycin selectively attenuates DNA-damage-induced neuronal cell death.
Authors: Makarevich, Oleg and Sabirzhanov, Boris and Aubrecht, Taryn G and Glaser, Ethan P and Polster, Brian M and Henry, Rebecca J and Faden, Alan I and Stoica, Bogdan A
Journal: Cell death & disease (2020): 587
Authors: Makarevich, Oleg and Sabirzhanov, Boris and Aubrecht, Taryn G and Glaser, Ethan P and Polster, Brian M and Henry, Rebecca J and Faden, Alan I and Stoica, Bogdan A
Journal: Cell death & disease (2020): 587
DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.
Authors: Shao, Zhengping and Flynn, Ryan A and Crowe, Jennifer L and Zhu, Yimeng and Liang, Jialiang and Jiang, Wenxia and Aryan, Fardin and Aoude, Patrick and Bertozzi, Carolyn R and Estes, Verna M and Lee, Brian J and Bhagat, Govind and Zha, Shan and Calo, Eliezer
Journal: Nature (2020): 291-296
Authors: Shao, Zhengping and Flynn, Ryan A and Crowe, Jennifer L and Zhu, Yimeng and Liang, Jialiang and Jiang, Wenxia and Aryan, Fardin and Aoude, Patrick and Bertozzi, Carolyn R and Estes, Verna M and Lee, Brian J and Bhagat, Govind and Zha, Shan and Calo, Eliezer
Journal: Nature (2020): 291-296
Acute aluminum chloride toxicity revisited: Study on DNA damage and histopathological, biochemical and neurochemical alterations in rat brain.
Authors: Liaquat, Laraib and Sadir, Sadia and Batool, Zehra and Tabassum, Saiqa and Shahzad, Sidrah and Afzal, Asia and Haider, Saida
Journal: Life sciences (2019): 202-211
Authors: Liaquat, Laraib and Sadir, Sadia and Batool, Zehra and Tabassum, Saiqa and Shahzad, Sidrah and Afzal, Asia and Haider, Saida
Journal: Life sciences (2019): 202-211
Selectivity of Terpyridine Platinum Anticancer Drugs for G-quadruplex DNA.
Authors: Morel, Elodie and Beauvineau, Claire and Naud-Martin, Delphine and Landras-Guetta, Corinne and Verga, Daniela and Ghosh, Deepanjan and Achelle, Sylvain and Mahuteau-Betzer, Florence and Bombard, Sophie and Teulade-Fichou, Marie-Paule
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Morel, Elodie and Beauvineau, Claire and Naud-Martin, Delphine and Landras-Guetta, Corinne and Verga, Daniela and Ghosh, Deepanjan and Achelle, Sylvain and Mahuteau-Betzer, Florence and Bombard, Sophie and Teulade-Fichou, Marie-Paule
Journal: Molecules (Basel, Switzerland) (2019)
DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells.
Authors: Liu, Dongjie and Li, Rui and Guo, Xianghua and Pang, Lijun and Zang, Yunjin and Liu, Kai and Chen, Dexi
Journal: Oncology letters (2018): 9333-9338
Authors: Liu, Dongjie and Li, Rui and Guo, Xianghua and Pang, Lijun and Zang, Yunjin and Liu, Kai and Chen, Dexi
Journal: Oncology letters (2018): 9333-9338
Regular exercise participation improves genomic stability in diabetic patients: an exploratory study to analyse telomere length and DNA damage.
Authors: Dimauro, Ivan and Sgura, Antonella and Pittaluga, Monica and Magi, Fiorenza and Fantini, Cristina and Mancinelli, Rosa and Sgadari, Antonio and Fulle, Stefania and Caporossi, Daniela
Journal: Scientific reports (2017): 4137
Authors: Dimauro, Ivan and Sgura, Antonella and Pittaluga, Monica and Magi, Fiorenza and Fantini, Cristina and Mancinelli, Rosa and Sgadari, Antonio and Fulle, Stefania and Caporossi, Daniela
Journal: Scientific reports (2017): 4137
Redox-linked effects of green tea on DNA damage and repair, and influence of microsatellite polymorphism in HMOX-1: results of a human intervention trial.
Authors: Choi, Siu-Wai and Yeung, Vincent T F and Collins, Andrew R and Benzie, Iris F F
Journal: Mutagenesis (2015): 129-37
Authors: Choi, Siu-Wai and Yeung, Vincent T F and Collins, Andrew R and Benzie, Iris F F
Journal: Mutagenesis (2015): 129-37