Calculator
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | N/A |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 498 |
| Emission (nm) | 522 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Real-Time PCR (qPCR)
Molecular weight N/A | Excitation (nm) 498 | Emission (nm) 522 |
SYBR Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
| VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
| ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
| JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
| Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
| SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
| ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
Show More (2) | |||
Images
Figure 1. ABI 7500 FAST system SYBR dye spectrum
References
View all 50 references: Citation Explorer
Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109
Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)
Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Overexpression of apoptosis-related protein, survivin, in fibroblasts from patients with systemic sclerosis.
Authors: Mahmoudi, Mohammad Bagher and Farashahi Yazd, Ehsan and Gharibdoost, Farhad and Sheikhha, Mohammad Hasan and Karimizadeh, Elham and Jamshidi, Ahmadreza and Mahmoudi, Mahdi
Journal: Irish journal of medical science (2019): 1443-1449
Authors: Mahmoudi, Mohammad Bagher and Farashahi Yazd, Ehsan and Gharibdoost, Farhad and Sheikhha, Mohammad Hasan and Karimizadeh, Elham and Jamshidi, Ahmadreza and Mahmoudi, Mahdi
Journal: Irish journal of medical science (2019): 1443-1449
Development of a new TaqMan PCR assay for the detection of both Entamoeba gingivalis genotypes.
Authors: Zaffino, Marie and Dubar, Marie and Debourgogne, Anne and Bisson, Catherine and Machouart, Marie
Journal: Diagnostic microbiology and infectious disease (2019): 114886
Authors: Zaffino, Marie and Dubar, Marie and Debourgogne, Anne and Bisson, Catherine and Machouart, Marie
Journal: Diagnostic microbiology and infectious disease (2019): 114886
A New Look at Causal Factors of Idiopathic Scoliosis: Altered Expression of Genes Controlling Chondroitin Sulfate Sulfation and Corresponding Changes in Protein Synthesis in Vertebral Body Growth Plates.
Authors: Zaydman, Alla M and Strokova, Elena L and O Stepanova, Alena and Laktionov, Pavel P and Shevchenko, Alexander I and Subbotin, Vladimir M
Journal: International journal of medical sciences (2019): 221-230
Authors: Zaydman, Alla M and Strokova, Elena L and O Stepanova, Alena and Laktionov, Pavel P and Shevchenko, Alexander I and Subbotin, Vladimir M
Journal: International journal of medical sciences (2019): 221-230
Application notes
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?