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AAT Bioquest

Is immunoprecipitation quantitative?

Posted May 26, 2021


Answer

Yes, immunoprecipitation is quantitative.

Immunoprecipitation (IP) is used to separate proteins that are bound to a specific antibody from the rest of a sample. the goal of immunoprecipitation is to isolate just enough protein to be able to measure it by western blotting or other semi-quantitative or quantitative assay methods. Usually treated and untreated samples are compared to assess the relative amount of the protein of interest.

Additional resources

Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins

ReadiLink™ xtra Rapid iFluor™ 350 Antibody Labeling Kit *BSA-Compatible*

Antibody and Protein Labeling