When are restriction enzymes used?

Restrictions enzymes are DNA-cutting enzymes. Different restriction enzymes recognize and cut different DNA sequences. The cuts are always made at a specific site. Restriction enzymes are a basic tool for biotechnology research. Isolated restriction enzymes are used to manipulate DNA for different scientific applications. They are used for DNA cloning and DNA fingerprinting. Restriction enzymes are also used to digest genomic DNA for gene analysis by Southern blot.