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AAT Bioquest

What are the steps of IR spectroscopy/vibrational spectroscopy?

Posted October 14, 2022


Answer

During IR spectroscopy/vibrational spectroscopy, compounds become analyzed by infrared radiation passing through the sample. This occurs over an array of different frequencies. The beam of infrared radiation is then reflected through a detector. The next step is measuring the absorptions created by each type of bond in the compound.   

There are three main ways a molecule can absorb radiation. Each of them involves an increase of energy that is proportional to the light absorbed. The first way occurs when absorption of radiation causes a higher rotational energy level in rotational transition. The second way is a vibrational transition that occurs on absorption of quantized energy (creates a higher vibrational energy level). The third way happens when electrons of molecules become raised to a high electron energy (known as electronic transition). 

Additional resources

INFRARED SPECTROSCOPY: A TOOL FOR DETERMINATION OF THE DEGREE OF CONVERSION IN DENTAL COMPOSITES