What laser excites DAPI?
Posted December 2, 2022
Answer
DAPI is excited by a UV 355 nm laser and captured with a 450/50 nm bandpass filter. DAPI can also be excited by the violet (405nm) laser line and detected within the blue fluorescence channel. The violet laser line is a novel technique, as the UV laser at 355 nm remains a high-priced laser.
Additional resources
DAPI - Blue Fluorescent DNA Stain
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*