Cal Green™ 1, hexapotassium salt

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Telephone	1-800-990-8053
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Physical properties

Dissociation constant (K_d, nM)	190
Molecular weight	1147.18
Solvent	Water

Spectral properties

Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.75¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal Green™ 1, hexapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.17 µL	435.851 µL	871.703 µL	4.359 mL	8.717 mL
5 mM	17.434 µL	87.17 µL	174.341 µL	871.703 µL	1.743 mL
10 mM	8.717 µL	43.585 µL	87.17 µL	435.851 µL	871.703 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.75¹

Images

Figure 1. Chemical structure for Cal Green™ 1, hexapotassium salt

References

View all 11 references: Citation Explorer

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
Authors: Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.
Journal: Nat Chem Biol (2007): 423

An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor
Authors: Lu Y, Paige MF.
Journal: J Fluoresc (2007): 739

Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green
Authors: Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.
Journal: Brain Res Brain Res Protoc (2000): 132

Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189

Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran
Authors: McPherson DR, McClellan AD, O'Donovan MJ.
Journal: Brain Res Brain Res Protoc (1997): 157

Modulation of an Intracellular Calmodulin-Stimulated Ca2+-Pumping ATPase in Cauliflower by Trypsin (The Use of Calcium Green-5N to Measure Ca2+ Transport in Membrane Vesicles)
Authors: Askerlund P., undefined
Journal: Plant Physiol (1996): 913

A method for recording intracellular [Ca2+] transients in cardiac myocytes using calcium green-2
Authors: Spencer CI, Berlin JR.
Journal: Pflugers Arch (1995): 579

Characterization of calcium translocation across the plasma membrane of primary osteoblasts using a lipophilic calcium-sensitive fluorescent dye, calcium green C18
Authors: Lloyd QP, Kuhn MA, Gay CV.
Journal: J Biol Chem (1995): 22445

Calcium green-5N, a novel fluorescent probe for monitoring high intracellular free Ca2+ concentrations associated with glutamate excitotoxicity in cultured rat brain neurons
Authors: Rajdev S, Reynolds IJ.
Journal: Neurosci Lett (1993): 149