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AAT Bioquest

Cal Green™ 1, hexapotassium salt

Cal Green™ 1 (AAT Bioquest) is the same molecule to Calcium Green-1 (Invitrogen). It exhibits an increase in fluorescence intensity upon binding calcium ion. The cell-impermeant dye, Cal Green 1 (Calcium Green-1) is a 488 nm-excitable calcium indicator. Compared to Fluo-3, Cal Green-1 is more fluorescent at low calcium concentrations, facilitating the determination of baseline calcium levels and increasing the visibility of resting cells.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal Green™ 1, hexapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.17 µL435.851 µL871.703 µL4.359 mL8.717 mL
5 mM17.434 µL87.17 µL174.341 µL871.703 µL1.743 mL
10 mM8.717 µL43.585 µL87.17 µL435.851 µL871.703 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 11 references: Citation Explorer
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor
Authors: Lu Y, Paige MF.
Journal: J Fluoresc (2007): 739
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
Authors: Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.
Journal: Nat Chem Biol (2007): 423
Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green
Authors: Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.
Journal: Brain Res Brain Res Protoc (2000): 132
Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran
Authors: McPherson DR, McClellan AD, O'Donovan MJ.
Journal: Brain Res Brain Res Protoc (1997): 157
Page updated on October 4, 2024

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Catalog Number20500
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Physical properties

Dissociation constant (Kd, nM)190

Molecular weight

1147.18

Solvent

Water

Spectral properties

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.751

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200