Name: Cal Green™ 1, hexapotassium salt
Brand: AAT Bioquest
SKU: 20500
Price: 174 USD
Availability: InStock

Cal Green™ 1, hexapotassium salt

Cal Green™ 1 (AAT Bioquest) is the same molecule to Calcium Green-1 (Invitrogen). It exhibits an increase in fluorescence intensity upon binding calcium ion. The cell-impermeant dye, Cal Green 1 (Calcium Green-1) is a 488 nm-excitable calcium indicator. Compared to Fluo-3, Cal Green-1 is more fluorescent at low calcium concentrations, facilitating the determination of baseline calcium levels and increasing the visibility of resting cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20500	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal Green™ 1, hexapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.17 µL	435.851 µL	871.703 µL	4.359 mL	8.717 mL
5 mM	17.434 µL	87.17 µL	174.341 µL	871.703 µL	1.743 mL
10 mM	8.717 µL	43.585 µL	87.17 µL	435.851 µL	871.703 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1147.18 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1

Authors:

Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.

Journal:

Methods Mol Biol (2011): 377

An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor

Authors:

Lu Y, Paige MF.

Journal:

J Fluoresc (2007): 739

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator

Authors:

Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.

Journal:

Nat Chem Biol (2007): 423

Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green

Authors:

Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.

Journal:

Brain Res Brain Res Protoc (2000): 132

Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran

Authors:

McPherson DR, McClellan AD, O'Donovan MJ.

Journal:

Brain Res Brain Res Protoc (1997): 157

Physical properties

Dissociation constant (K_d, nM)	190
Molecular weight	1147.18
Solvent	Water

Spectral properties

Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on September 26, 2025