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Cal Green™ 1, hexapotassium salt
Cal Green™ 1 (AAT Bioquest) is the same molecule to Calcium Green-1 (Invitrogen). It exhibits an increase in fluorescence intensity upon binding calcium ion. The cell-impermeant dye, Cal Green 1 (Calcium Green-1) is a 488 nm-excitable calcium indicator. Compared to Fluo-3, Cal Green-1 is more fluorescent at low calcium concentrations, facilitating the determination of baseline calcium levels and increasing the visibility of resting cells.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal Green™ 1, hexapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 87.17 µL | 435.851 µL | 871.703 µL | 4.359 mL | 8.717 mL |
| 5 mM | 17.434 µL | 87.17 µL | 174.341 µL | 871.703 µL | 1.743 mL |
| 10 mM | 8.717 µL | 43.585 µL | 87.17 µL | 435.851 µL | 871.703 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
References
View all 11 references: Citation Explorer
Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377
An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor
Authors: Lu Y, Paige MF.
Journal: J Fluoresc (2007): 739
Authors: Lu Y, Paige MF.
Journal: J Fluoresc (2007): 739
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
Authors: Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.
Journal: Nat Chem Biol (2007): 423
Authors: Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.
Journal: Nat Chem Biol (2007): 423
Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green
Authors: Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.
Journal: Brain Res Brain Res Protoc (2000): 132
Authors: Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.
Journal: Brain Res Brain Res Protoc (2000): 132
Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran
Authors: McPherson DR, McClellan AD, O'Donovan MJ.
Journal: Brain Res Brain Res Protoc (1997): 157
Authors: McPherson DR, McClellan AD, O'Donovan MJ.
Journal: Brain Res Brain Res Protoc (1997): 157
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