Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]

Cal Green™ 1, AM

Equivalent to Calcium Green-1, AM

Cal Green™ 1 (AAT Bioquest) is the same molecule to Calcium Green-1 (Invitrogen). It exhibits an increase in fluorescence intensity upon binding calcium ion. The cell-permeant dye, Cal Green 1 AM (Calcium Green-1 AM) is a 488 nm-excitable calcium indicator. Compared to Fluo-3 AM, Cal Green-1 AM is more fluorescent at low calcium concentrations in cells, facilitating the determination of baseline calcium levels and increasing the visibility of resting cells. It has been used in many calcium signaling investigations, including measuring intracellular calcium, following calcium influx and release, and multiphoton excitation imaging of calcium in living tissues. Cells can be loaded with Cal Green-1 AM by adding the dissolved indicator directly to cultured cells in medium. The fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry.

Protocol

COA

Catalog	Size	Price	Quantity
20501	10x50 ug	Price
20502	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	77.462 µL	387.309 µL	774.617 µL	3.873 mL	7.746 mL
5 mM	15.492 µL	77.462 µL	154.923 µL	774.617 µL	1.549 mL
10 mM	7.746 µL	38.731 µL	77.462 µL	387.309 µL	774.617 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1290.96 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery

Authors:

Zhang, Nisi and Guo, Yutong and Foiret, Josquin and Tumbale, Spencer K and Paulmurugan, Ramasamy and Ferrara, Katherine W

Journal:

STAR protocols (2023): 102723

Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia

Authors:

Jiang, Xiao-wen and Lu, Hong-yuan and Xu, Zi-Hua and Zhang, Ying-Shi and Zhao, Qing-Chun and others,

Journal:

Fitoterapia (2021): 104824

Binding to carboxypeptidase M mediates protective effects of fibrinopeptide Bβ (15-42)

Authors:

Sörensen-Zender, Inga and Chen, Rongjun and Song, Rong and David, Sascha and Melk, Anette and Haller, Hermann and Schmitt, Rol and , undefined

Journal:

Translational Research (2019)

Tuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores

Authors:

Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J

Journal:

ACS chemical biology (2017): 1844--1852

Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores

Authors:

Jia, Shang and Ramos-Torres, Karla M and Kolemen, Safacan and Ackerman, Cheri M and Chang, Christopher J

References

View all 11 references: Citation Explorer

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1

Authors:

Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.

Journal:

Methods Mol Biol (2011): 377

An ensemble and single-molecule fluorescence spectroscopy investigation of Calcium Green 1, a calcium-ion sensor

Authors:

Lu Y, Paige MF.

Journal:

J Fluoresc (2007): 739

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator

Authors:

Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY.

Journal:

Nat Chem Biol (2007): 423

Measurement of intracellular calcium levels by the fluorescent Ca2+ indicator Calcium-Green

Authors:

Silei V, Fabrizi C, Venturini G, Tagliavini F, Salmona M, Bugiani O, Lauro GM.

Journal:

Brain Res Brain Res Protoc (2000): 132

Optical imaging of neuronal activity in tissue labeled by retrograde transport of Calcium Green Dextran

Authors:

McPherson DR, McClellan AD, O'Donovan MJ.

Journal:

Brain Res Brain Res Protoc (1997): 157

Physical properties

Dissociation constant (K_d, nM)	190
Molecular weight	1290.96
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	186501-28-0

Instrument settings

Fluorescence microscope
Excitation	FITC
Emission	FITC
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025