Rhod-2, tripotassium salt

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Physical properties

Dissociation constant (K_d, nM)	570
Molecular weight	869.05
Solvent	Water

Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

869.05

Dissociation constant (K_d, nM)

570

Excitation (nm)

553

Emission (nm)

577

Quantum yield

0.1¹

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	115.068 µL	575.341 µL	1.151 mL	5.753 mL	11.507 mL
5 mM	23.014 µL	115.068 µL	230.136 µL	1.151 mL	2.301 mL
10 mM	11.507 µL	57.534 µL	115.068 µL	575.341 µL	1.151 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1¹

Product Family

Name	Excitation (nm)	Emission (nm)
Rhod-2, trisodium salt	553	577
Rhod-5N, tripotassium salt	557	580
Rhod-FF, tripotassium salt	553	577

Images

Figure 1. Chemical structure for Rhod-2, tripotassium salt

Citations

View all 13 citations: Citation Explorer

Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108

Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074

Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8

GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)

BAX inhibitor-1 is a Ca2&plus; channel critically important for immune cell function and survival
Authors: Lisak, D and Schacht, T and Gawlitza, A and Albrecht, P and Aktas, O and Koop, B and Gliem, M and Hofstetter, HH and Zanger, K and Bultynck, Geert and others, undefined
Journal: Cell Death & Differentiation (2016): 358--368

Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells
Authors: Shen, Luyan and Wen, Naiyan and Xia, Meihui and Zhang, Yu and Liu, Weimin and Xu, Ye and Sun, Liankun
Journal: Oncology letters (2016): 2411--2419

Failure of Elevating Calcium Induces Oxidative Stress Tolerance and Imparts Cisplatin Resistance in Ovarian Cancer Cells
Authors: Ma, Liwei and Wang, Hongjun and Wang, Chunyan and Su, Jing and Xie, Qi and Xu, Lu and Yu, Yang and Liu, Shibing and Li, Songyan and Xu, Ye and others, undefined
Journal: Aging and Disease (2016): 254

ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells
Authors: YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN
Journal: International Journal of Oncology (2016): 2507--2519

Voltage and calcium dual channel optical mapping of cultured HL-1 atrial myocyte monolayer
Authors: Yan, Jiajie and Thomson, Justin K and Zhao, Weiwei and Fast, Vladimir G and Ye, Tong and Ai, Xun
Journal: JoVE (Journal of Visualized Experiments) (2015): e52542--e52542

Cardiac tissue slices: preparation, handling, and successful optical mapping
Authors: Wang, Ken and Lee, Peter and Mirams, Gary R and Sarathch, undefined and ra, Padmini and Borg, Thomas K and Gavaghan, David J and Kohl, Peter and Bollensdorff, Christian
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2015): H1112--H1125