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Rhod-2, tripotassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM115.068 µL575.341 µL1.151 mL5.753 mL11.507 mL
5 mM23.014 µL115.068 µL230.136 µL1.151 mL2.301 mL
10 mM11.507 µL57.534 µL115.068 µL575.341 µL1.151 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)
Rhod-2, trisodium salt553577
Rhod-5N, tripotassium salt557580
Rhod-FF, tripotassium salt553577

Citations

View all 13 citations: Citation Explorer
GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074
Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108
ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells
Authors: YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN
Journal: International Journal of Oncology (2016): 2507--2519
Page updated on December 13, 2024

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Catalog Number21067
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Physical properties

Dissociation constant (Kd, nM)570

Molecular weight

869.05

Solvent

Water

Spectral properties

Excitation (nm)

553

Emission (nm)

577

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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