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Rhod-2, tripotassium salt
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Application notes
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, tripotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 115.068 µL | 575.341 µL | 1.151 mL | 5.753 mL | 11.507 mL |
| 5 mM | 23.014 µL | 115.068 µL | 230.136 µL | 1.151 mL | 2.301 mL |
| 10 mM | 11.507 µL | 57.534 µL | 115.068 µL | 575.341 µL | 1.151 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Rhod-2, trisodium salt | 553 | 577 |
| Rhod-5N, tripotassium salt | 557 | 580 |
| Rhod-FF, tripotassium salt | 553 | 577 |
Citations
View all 13 citations: Citation Explorer
GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074
Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108
ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells
Authors: YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN
Journal: International Journal of Oncology (2016): 2507--2519
Authors: YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN
Journal: International Journal of Oncology (2016): 2507--2519