Rhod-2, trisodium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21068	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, trisodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.843 µL	609.214 µL	1.218 mL	6.092 mL	12.184 mL
5 mM	24.369 µL	121.843 µL	243.685 µL	1.218 mL	2.437 mL
10 mM	12.184 µL	60.921 µL	121.843 µL	609.214 µL	1.218 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	820.73 g/mol	=	mL	x	mM	=	mmol

Citations

View all 13 citations: Citation Explorer

GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction

Authors:

Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin

Journal:

Brain Research Bulletin (2017)

Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression

Authors:

Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue

Journal:

Journal of Biosciences and Medicines (2017): 8

Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells

Authors:

Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined

Journal:

PLoS Negl Trop Dis (2017): e0006074

Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans

Authors:

Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP

Journal:

Biophysical Journal (2017): 1093--1108

ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells

Authors:

YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN

Journal:

International Journal of Oncology (2016): 2507--2519

Physical properties

Dissociation constant (K_d, nM)	570
Molecular weight	820.73
Solvent	Water

Spectral properties

Excitation (nm)	553
Emission (nm)	577
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 3, 2025