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Rhod-2, trisodium salt

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Physical properties
Dissociation constant (Kd, nM)570
Molecular weight820.73
Spectral properties
Excitation (nm)553
Emission (nm)577
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Dissociation constant (Kd, nM)
Excitation (nm)
Emission (nm)
Quantum yield
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, trisodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM121.843 µL609.214 µL1.218 mL6.092 mL12.184 mL
5 mM24.369 µL121.843 µL243.685 µL1.218 mL2.437 mL
10 mM12.184 µL60.921 µL121.843 µL609.214 µL1.218 mL

Molarity calculator

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Spectral properties

Excitation (nm)553
Emission (nm)577
Quantum yield0.11

Product Family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-2, tripotassium salt5535770.11



View all 13 citations: Citation Explorer
Spatially Organized &beta;-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108
Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)
BAX inhibitor-1 is a Ca2&amp;plus; channel critically important for immune cell function and survival
Authors: Lisak, D and Schacht, T and Gawlitza, A and Albrecht, P and Aktas, O and Koop, B and Gliem, M and Hofstetter, HH and Zanger, K and Bultynck, Geert and others, undefined
Journal: Cell Death &amp; Differentiation (2016): 358--368
Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells
Authors: Shen, Luyan and Wen, Naiyan and Xia, Meihui and Zhang, Yu and Liu, Weimin and Xu, Ye and Sun, Liankun
Journal: Oncology letters (2016): 2411--2419
Failure of Elevating Calcium Induces Oxidative Stress Tolerance and Imparts Cisplatin Resistance in Ovarian Cancer Cells
Authors: Ma, Liwei and Wang, Hongjun and Wang, Chunyan and Su, Jing and Xie, Qi and Xu, Lu and Yu, Yang and Liu, Shibing and Li, Songyan and Xu, Ye and others, undefined
Journal: Aging and Disease (2016): 254
ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells
Journal: International Journal of Oncology (2016): 2507--2519
Voltage and calcium dual channel optical mapping of cultured HL-1 atrial myocyte monolayer
Authors: Yan, Jiajie and Thomson, Justin K and Zhao, Weiwei and Fast, Vladimir G and Ye, Tong and Ai, Xun
Journal: JoVE (Journal of Visualized Experiments) (2015): e52542--e52542
Cardiac tissue slices: preparation, handling, and successful optical mapping
Authors: Wang, Ken and Lee, Peter and Mirams, Gary R and Sarathch, undefined and ra, Padmini and Borg, Thomas K and Gavaghan, David J and Kohl, Peter and Bollensdorff, Christian
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2015): H1112--H1125