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Rhod-2, trisodium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. The long-wavelength Rhod-2 Ca2+ indicators are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high levels of autofluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-2, trisodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM121.843 µL609.214 µL1.218 mL6.092 mL12.184 mL
5 mM24.369 µL121.843 µL243.685 µL1.218 mL2.437 mL
10 mM12.184 µL60.921 µL121.843 µL609.214 µL1.218 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-2, tripotassium salt5535770.11

Citations

View all 13 citations: Citation Explorer
GluR3B Ab's induced oligodendrocyte precursor cells excitotoxicity via mitochondrial dysfunction
Authors: Liu, Yi and Chen, Yan and Du, Wan Tong and Wu, Xiu Xiang and Dong, Fu Xing and Qu, Xue Bin and Fan, Hong Bin and Yao, Rui Qin
Journal: Brain Research Bulletin (2017)
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
Csseverin inhibits apoptosis through mitochondria-mediated pathways triggered by Ca2+ dyshomeostasis in hepatocarcinoma PLC cells
Authors: Shi, M and Zhou, L and Zhao, L and Shang, M and He, T and Tang, Z and others, undefined
Journal: PLoS Negl Trop Dis (2017): e0006074
Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans
Authors: Westacott, Matthew J and Ludin, Nurin WF and Benninger, Richard KP
Journal: Biophysical Journal (2017): 1093--1108
ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells
Authors: YU, CHUNYAN and JIANG, XIANRUI and XU, YE and SUN, LIANKUN
Journal: International Journal of Oncology (2016): 2507--2519
Page updated on December 6, 2024

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Catalog Number21068
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Physical properties

Dissociation constant (Kd, nM)570

Molecular weight

820.73

Solvent

Water

Spectral properties

Excitation (nm)

553

Emission (nm)

577

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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