AATOM™ dyes are high-performance fluorophores characterized by high photostability and brightness, making them suitable for demanding fluorescence applications including oligonucleotide labeling, single-molecule fluorescence studies, and advanced microscopy techniques. These rhodamine and carbopyronine derivatives exhibit quantum yields exceeding 0.8 for many variants while demonstrating strong resistance to photobleaching.

The product line spans 390–700 nm excitation. These dyes are commonly used in oligonucleotide labeling applications, where photostability during repeated imaging cycles is critical. AATOM™ 488, AATOM™ 565, and AATOM™ 647N are widely used for FISH probes, molecular beacons, and qPCR applications. For super-resolution microscopy, AATOM™ 647N and AATOM™ 655 exhibit the photoswitching behavior required for STORM/dSTORM techniques.

AATOM™ dyes are manufactured by AAT Bioquest and are not affiliated with ATTO-TEC GmbH.

Oligonucleotide Labeling: NHS esters and phosphoramidite-compatible derivatives enable direct incorporation during or after synthesis. The exceptional photostability ensures consistent signal across extended imaging sessions, which is beneficial for repeated imaging and time-lapse studies.

Single-Molecule Detection: High extinction coefficients (>100,000 M⁻¹cm⁻¹ for most variants) combined with high quantum yields deliver the brightness required for single-molecule FRET, FCS, and single-particle tracking.

Super-Resolution Microscopy: AATOM™ 647N, AATOM™ 655, and AATOM™ 680 are established choices for dSTORM due to their efficient photoswitching in thiol-containing imaging buffers (AATOM™ 655/680) and β‑mercaptoethanol (AATOM™ 647N).

Multiplexing: The spectral range from violet (AATOM™ 390) through near-IR (AATOM™ 700) enables multicolor experiments with minimal crosstalk when paired with appropriate filter sets and instrumentation.

Both dyes share similar excitation/emission profiles but differ significantly in performance characteristics:

AATOM™ 647N is the carbopyronine derivative with dramatically improved quantum yield and photostability. For many applications, AATOM™ 647N is often preferred, although performance requirements and cost considerations may vary by application.