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APC-CD Antibody Conjugates
APC (Allophycocyanin) antibody conjugates are essential reagents for flow cytometry and immunofluorescence applications, offering bright far-red fluorescence with excitation at 633-647 nm and emission around 660 nm. AAT Bioquest provides a comprehensive selection of APC and APC tandem dye conjugated CD antibodies for immunophenotyping, cell surface marker analysis, and multicolor flow cytometry panels. These conjugates are optimized for compatibility with common laser configurations and can be combined with other fluorophores for sophisticated multiparameter analysis.
CD Marker | Synonyms | Primary Cell Types | Common Applications |
|---|---|---|---|
CD3 | T3 | T lymphocytes | Pan-T cell marker, T cell activation studies |
CD4 | T4, Leu-3 | Helper T cells, monocytes | T helper cell identification, HIV research, immunocompetence assessment |
CD8 | T8, Leu-2 | Cytotoxic T lymphocytes, NK cells | CTL identification, immune monitoring, tumor-infiltrating lymphocyte analysis |
CD14 | LPS receptor, Monocyte marker | Monocytes, macrophages | Monocyte gating, inflammation studies, bacterial response assessment |
CD19 | B4 | B lymphocytes | Pan-B cell marker, lymphoma typing, B cell lymphoma classification |
CD20 | B1, Bp35 | B lymphocytes | B cell development, rituximab therapy monitoring, B cell malignancy detection |
CD25 | IL-2Ralpha | Activated T cells, regulatory T cells (Tregs) | Regulatory T cell identification, T cell activation marker, immunosuppression studies |
CD34 | Mucosialin | Hematopoietic stem and progenitor cells | Stem cell enumeration, transplant monitoring, hematopoietic assessment |
CD45 | LCA (Leukocyte Common Antigen) | All leukocytes | Pan-leukocyte gating, lymphoma diagnosis, immune cell enumeration |
CD56 | NCAM (Neural Cell Adhesion Molecule) | Natural killer (NK) cells, NKT cells | NK cell identification, immunotherapy monitoring, cancer immunology studies |
APC and APC Tandem Conjugated CD Antibodies
APC (Allophycocyanin) is a phycobiliprotein derived from cyanobacteria and red algae that provides exceptional brightness in the far-red spectrum. With excitation at 633-647 nm (red laser) and emission at approximately 660 nm, APC conjugates are ideal for multicolor flow cytometry panels where spectral separation from PE and FITC channels is required. APC is among the brightest single fluorophores used in flow cytometry, providing strong far-red signals when excited by standard red lasers.
- Exceptional brightness - High extinction coefficient and quantum yield for superior signal detection
- Red laser compatible - Optimal excitation at 633-647 nm, standard on most flow cytometers
- Minimal spectral overlap - Far-red emission (660 nm) allows clean separation from PE, FITC, and other common fluorophores
- Ideal for multicolor panels - Pairs well with APC tandems (APC-Cy7, APC-iFluor® 700) for expanded far-red detection
- Low background - Reduced cellular autofluorescence in the far-red spectrum improves signal-to-noise ratio
Building Multicolor Flow Cytometry Panels
For comprehensive immunophenotyping experiments, APC-CD antibody conjugates can be combined with other fluorophore-conjugated antibodies to create multicolor panels. AAT Bioquest offers APC tandem dyes that expand the far-red detection options available from a single red laser.
- APC tandems available - APC-Cy7 and other tandems for additional far-red channels
- Optimized for panel design - Consistent lot-to-lot performance for reproducible results
- ReadiUse format available - Pre-optimized, ready-to-use formulations for convenience
An example panel can be found in the table below:
Fluorophore | Ex/Em (nm) | Laser | Common Filter | Panel Position |
|---|---|---|---|---|
APC | 650/660 | Red (633-647 nm) | 660/20 | High-expression markers |
APC-Cy7 | 650/785 | Red (633-647 nm) | 780/60 | Dump channel or high-expression |
PE | 496/578 | Blue/Yellow-Green | 575/26 | Low-expression markers |
FITC | 494/520 | Blue (488 nm) | 530/30 | Medium-expression markers |
Custom Conjugation Solutions
For researchers requiring specific APC-CD antibody conjugates not currently in the catalog, AAT Bioquest offers Buccutite™ conjugation technology that enables efficient, site-specific labeling of antibodies with APC and other phycobiliproteins. Explore our Antibody Conjugation and Labeling Services or browse Custom Labeling Options.
- Higher conjugation efficiency - Buccutite™ chemistry provides superior yields compared to traditional SMCC methods
- Optimized for phycobiliproteins - Specially designed for APC, PE, and tandem dye conjugations
- Consistent performance - Reproducible results across multiple conjugation batches
This document (01.0283.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.