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Calcium Assays
AAT Bioquest offers a comprehensive portfolio of calcium assay kits and reagents designed for high-throughput screening (HTS) applications. These products enable sensitive detection of intracellular calcium mobilization for GPCR screening, ion channel studies, and cell signaling research. Our Screen Quest™ assay kits feature optimized formulations with proprietary calcium indicators including Calbryte™, Fluo-8®, and Rhod-4, providing superior signal-to-background ratios and streamlined workflows for microplate readers, FLIPR systems, and flow cytometry platforms.
Screen Quest™ Calcium Assay Kits
Screen Quest™ calcium assay kits are optimized for high-throughput screening of GPCRs, ion channels, and other calcium-dependent signaling pathways. These complete kits include proprietary calcium indicators, optimized buffers, and signal enhancers formulated for maximum assay windows on FLIPR, FlexStation, and standard microplate readers. Multiple indicator options provide flexibility in excitation/emission wavelengths to match instrument configurations and multiplexing requirements.
For standalone calcium detection reagents, please see our calcium indicators catalog.
Key Features
- No-wash homogeneous formats eliminate washing steps for true walk-away automation
- Probenecid-free options available for sensitive cell lines and reduced cytotoxicity concerns
- Optimized signal enhancers maximize fluorescence intensity and assay window
- Compatible with FLIPR, FlexStation, and standard plate readers for kinetic or endpoint measurements
- Superior Calbryte™ and Fluo-8® indicators provide brighter signals than Fluo-3 and Fluo-4
Fig. 1
Comparison of homogeneous calcium assays on response of endogenous P2Y receptor to ATP in CHO-M1 cells in the absence of probenecid. CHO-M1 cells were seeded overnight in 50,000 cells per 100 µl per well in a 96-well black wall/clear bottom costar plate. The cells were incubated with 100 µl of Cal 520™ PBC-free Ca Kit, Cal 520™ NW kit, BD™ PBX Ca kit, Fluo-4 direct or Calcium 4 in HHBS with or without 1 or 2.5 mM probenecid for 2 hours at 37oC, 5% CO2 incubator. ATP (50 µL/well) was added using Flexstation to achieve the final indicated concentrations.
This document (01.0274.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Calcium Assays