AAT Bioquest offers a comprehensive selection of CPG (Controlled Pore Glass) reagents for oligonucleotide synthesis, enabling researchers to incorporate fluorescent labels, dark quenchers, and specialty modifications directly at the 3'-terminus during solid-phase synthesis. Our portfolio includes fluorescein and TAMRA dyes for detection, the superior Tide Fluor™ and Tide Quencher™ series for FRET applications, AzoDye quenchers, minor groove binders for enhanced probe performance, and specialty reagents for unique oligonucleotide modifications. Most of these products are available in both 500 Å and 1000 Å pore sizes to accommodate different oligonucleotide lengths.

Fluorescent dye CPGs enable direct incorporation of fluorophore labels at the 3'-terminus during oligonucleotide synthesis. These solid supports are pre-loaded with fluorescent dyes including fluorescein variants (FAM, FAM-xtra), TAMRA, and the superior Tide Fluor™ 1 (TF1). FAM-xtra offers improved performance with identical spectral properties to standard FAM, while TF1 provides significantly enhanced brightness compared to EDANS for UV-excitable applications.

Tide Quencher™ CPGs are high-performance dark quencher solid supports for synthesizing FRET-based oligonucleotide probes. The complete TQ1–TQ8 series provides spectral coverage from UV through near-infrared, enabling optimal quenching of virtually any fluorophore. These non-fluorescent quenchers feature stronger absorption and higher quenching efficiency compared to traditional quenchers like DABCYL, BHQ, and Eclipse.

AzoDye CPGs and DABCYL CPG are dark quencher solid supports for FRET oligonucleotide synthesis. AzoDye quenchers are nearly non-fluorescent with moderate extinction coefficients, making them effective quenchers for various fluorophore-labeled probes. DABCYL remains one of the most widely used traditional dark quenchers for diagnostic probe applications.

In addition to fluorescent dyes and dark quenchers, specialty CPG reagents are offered for unique oligonucleotide modifications. CDPI3-CPG (Minor Groove Binder) enhances probe performance by binding to the DNA minor groove, increasing duplex stability and hybridization specificity, which is particularly valuable for short probes and SNP detection. Additional specialty reagents include Chemical Phosphorylation Reagent I for adding 3'-phosphate groups and 3'-Uaq Cap CPG for enhanced duplex stability through anthraquinone-mediated interactions.