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Goat Anti-Human Secondary Antibodies
AAT Bioquest offers a comprehensive selection of goat anti-human secondary antibodies designed for sensitive and specific detection of human immunoglobulins in research applications. This collection includes iFluor® dye conjugates, XFD (Alexa Fluor® replacement) conjugates, phycobiliprotein conjugates, enzyme conjugates, and innovative PSA (Power Styramide™ Signal Amplification) imaging kits for ultrasensitive immunofluorescence detection.
Detection Method | Product Types | Best For | Key Advantage |
|---|---|---|---|
Direct fluorescence | iFluor®, XFD, Phycobiliprotein and other classic dye conjugates | Flow cytometry, IF microscopy, multiplexing | Simple protocol, wide spectral range |
Enzymatic amplification | HRP, ALP conjugates | ELISA, Western blot, IHC | Signal amplification, versatile substrates |
Biotin-streptavidin systems | Biotinylated conjugates | Signal amplification, flexible detection | Compatible with any streptavidin conjugate |
Power Styramide™ Signal Amplification | PSA imaging kits | Low-abundance targets, IHC | 10-100× signal enhancement |
Direct Fluorescence Detection
Direct fluorescence detection uses fluorophore-conjugated secondary antibodies for immediate visualization without additional amplification steps. AAT Bioquest offers multiple fluorophore platforms including proprietary iFluor® dyes, XFD (Alexa Fluor® replacement) dyes, phycobiliproteins (PE/APC), and classic fluorophores to match any instrument configuration.
iFluor® Conjugates
iFluor® dyes represent AAT Bioquest's next-generation fluorophores, offering superior brightness and photostability compared to traditional dyes. Goat anti-human IgG antibodies conjugated to iFluor® dyes are ideal for demanding applications requiring maximum signal intensity.
Key Features
- Superior brightness – Up to 2× brighter than comparable Alexa Fluor® dyes
- Enhanced photostability – Extended imaging without significant signal loss
- Full spectral coverage – Options from UV to near-infrared
- Optimized F/P ratios – Consistent lot-to-lot performance
XFD (Alexa Fluor® Replacement) Conjugates
XFD dyes are spectrally equivalent to Alexa Fluor® dyes, providing a cost-effective alternative with identical excitation/emission profiles. These conjugates are compatible with existing filter sets and can be used as drop-in replacements in established protocols.
Key Features
- Spectral equivalence – Same Ex/Em as corresponding Alexa Fluor® dyes
- Filter compatibility – Works with existing microscope and cytometer configurations
- Cost-effective – Premium performance at competitive pricing
- Validated performance – Tested for IHC, IF, flow cytometry, and Western blot
Phycobiliprotein Conjugates
Phycobiliprotein-conjugated secondary antibodies (PE, APC, and tandem dyes) offer exceptional brightness for flow cytometry applications. These conjugates are particularly useful for detecting low-abundance antigens and building multicolor immunophenotyping panels.
Key Features
- Exceptional brightness – Phycobiliproteins are among the brightest biological fluorophores
- Flow cytometry optimized – Ideal for multicolor panel design
- Tandem options – Extended spectral range with PE and APC tandems
- Low background – Minimal non-specific binding
Classic Dye Conjugates
Classic fluorescent dye conjugates including Pacific Blue, Pacific Orange, FITC, and Cy dyes remain essential tools for routine immunofluorescence and flow cytometry applications.
Enzymatic Amplification (HRP and ALP Conjugates)
Enzyme-conjugated secondary antibodies enable signal amplification through enzymatic substrate conversion. HRP (horseradish peroxidase) and ALP (alkaline phosphatase) conjugates are essential for colorimetric, fluorescent, and chemiluminescent detection in ELISA, Western blot, and immunohistochemistry applications.
Key Features
- Signal amplification – Enzymatic turnover generates intense detectable signal
- Versatile detection – Compatible with colorimetric, fluorescent, and chemiluminescent substrates
- ELISA optimized – Validated for quantitative immunoassays
- IHC/ICC compatible – Suitable for tissue and cell staining applications
Biotin-Streptavidin Systems
Biotinylated secondary antibodies leverage the high-affinity biotin-streptavidin interaction for flexible, amplifiable detection. These conjugates can be paired with any streptavidin-conjugated reporter for customized detection workflows.
Key Features
- Flexible detection – Compatible with any streptavidin conjugate
- Signal amplification – Multiple biotin molecules per antibody enhance sensitivity
- Multiplexing compatible – Use with different streptavidin conjugates for multicolor detection
- Universal platform – Works across ELISA, IHC, IF, and flow cytometry
Unconjugated Antibodies
Unconjugated goat anti-human IgG antibodies provide maximum flexibility for researchers who need to perform custom conjugations or require unlabeled antibodies for specialized protocols.
Power Styramide™ Signal Amplification (PSA)—Next-Generation TSA
Power Styramide™ Signal Amplification (PSA) delivers up to 50x greater sensitivity than traditional tyramide signal amplification (TSA), enabling detection of low-abundance targets that conventional immunofluorescence methods often miss. PSA combines two key innovations: Styramide™ substrates with significantly higher reactivity than tyramide, and iFluor® dyes with exceptional brightness and photostability. The result is dramatically enhanced fluorescent signals that remain stable through extended imaging sessions, stringent washes, and multiplex protocols.
- Broad application compatibility: Optimized for IHC (including FFPE tissues), ICC, and in situ hybridization (ISH)
- Multiplex-ready: Sequential staining with spectrally distinct PSA kits enables multi-target detection, even with same-species primary antibodies
- Complete kit format: Each kit includes HRP-conjugated goat anti-human IgG secondary antibody and iFluor®-labeled Styramide™ reagent for ~100 tests
- Drop-in workflow: Uses the same HRP conjugates, buffers, and protocol steps as traditional TSA
Why PSA Outperforms Traditional TSA
Feature | PSA (Power Styramide™) | Traditional TSA |
|---|---|---|
Sensitivity | Up to 50x greater signal | Baseline |
Substrate reactivity | Styramide™ radicals—faster, more efficient covalent deposition | Tyramide radicals |
Fluorophore performance | iFluor® dyes—superior brightness, photostability, and thermal resistance | Alexa Fluor or equivalent |
Primary antibody usage | 10-50x lower concentrations | Standard concentrations |
Protocol compatibility | Drop-in replacement—same HRP conjugates, buffers, and workflow | — |
This document (01.0286.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Goat Anti-Human Secondary Antibodies