iFluor®-CD Antibody Conjugates

AAT Bioquest offers a comprehensive selection of iFluor®-CD antibody conjugates designed for immunophenotyping and cell surface marker analysis. These pre-conjugated antibodies combine the superior brightness and photostability of iFluor® dyes with validated antibodies against cluster of differentiation (CD) markers, providing ready-to-use reagents for flow cytometry and immunofluorescence applications. The iFluor® dye series spans from ultraviolet (UV) through visible to near-infrared (NIR) wavelengths, enabling flexible multicolor panel design.

iFluor® Dye Technology

iFluor® dyes represent AAT Bioquest's next-generation fluorescent labeling technology, engineered to overcome limitations of traditional fluorophores like FITC, Cy dyes, and Alexa Fluor® dyes. When conjugated to CD antibodies, iFluor® dyes deliver exceptional signal intensity and photostability for reliable immunophenotyping across flow cytometry and microscopy platforms.

Key Advantages

Superior brightness - iFluor® dyes provide 2-5X higher fluorescence intensity than comparable FITC and Cy dye conjugates
Enhanced photostability - Significantly more resistant to photobleaching than Alexa Fluor® and Cy dyes, enabling extended imaging sessions and repeated scanning without signal loss
Higher conjugation efficiency - Optimized reactive chemistry yields more consistent dye-to-protein ratios and reduced dye aggregation
Excellent water solubility - Superior aqueous solubility minimizes aggregation and non-specific binding in cellular assays
pH stability - Stable fluorescence across physiological pH range (pH 4-10) for diverse experimental conditions
Broad spectral coverage - Full visible to near-IR spectrum options for flexible multicolor panel design

CD Marker Profiles

CD (Cluster of Differentiation) antibody conjugates are essential tools for identifying and characterizing immune cell populations through flow cytometry. AAT Bioquest's iFluor®-CD conjugates target key markers across multiple cell lineages and differentiation states.

Antibody Quality Standards

Pre-validated specificity - Each conjugate is tested for specificity and performance in flow cytometry
Ready-to-use format - No additional conjugation steps required; directly applicable to cell suspensions
Optimized fluorophore-to-protein ratios - Balanced ratios maximize signal without introducing quenching or aggregation
Multiplatform compatibility - Validated for flow cytometry, immunofluorescence, and imaging applications
Multiple species reactivity - Human and mouse-reactive options available for cross-species studies

CD Marker	Synonyms	Primary Cell Types	Common Applications
CD3	T3	T lymphocytes	Pan-T cell marker, T cell activation studies
CD4	T4, Leu-3	Helper T cells, monocytes	T helper cell identification, HIV research, immunocompetence assessment
CD8	T8, Leu-2	Cytotoxic T lymphocytes, NK cells	CTL identification, immune monitoring, tumor-infiltrating lymphocyte analysis
CD14	LPS receptor, Monocyte marker	Monocytes, macrophages	Monocyte gating, inflammation studies, bacterial response assessment
CD19	B4	B lymphocytes	Pan-B cell marker, lymphoma typing, B cell lymphoma classification
CD20	B1, Bp35	B lymphocytes	B cell development, rituximab therapy monitoring, B cell malignancy detection
CD25	IL-2Ralpha	Activated T cells, regulatory T cells (Tregs)	Regulatory T cell identification, T cell activation marker, immunosuppression studies
CD34	Mucosialin	Hematopoietic stem and progenitor cells	Stem cell enumeration, transplant monitoring, hematopoietic assessment
CD45	LCA (Leukocyte Common Antigen)	All leukocytes	Pan-leukocyte gating, lymphoma diagnosis, immune cell enumeration
CD56	NCAM (Neural Cell Adhesion Molecule)	Natural killer (NK) cells, NKT cells	NK cell identification, immunotherapy monitoring, cancer immunology studies

iFluor®-CD Antibody Product Tables

The tables below list all available iFluor® dye-CD antibody conjugates organized by CD target and spectral range. Click any clone name to view the product page.

Example Immunophenotyping Panel

This basic T cell panel demonstrates optimal iFluor® dye selection for standard three-color analysis of T cell subsets:

iFluor® Dye	CD Marker	Cell Population Identified
iFluor® 488	CD3	Total T lymphocytes
iFluor® 555	CD4	Helper T cells (Th)
iFluor® 647	CD8	Cytotoxic T lymphocytes (CTL)
iFluor® 750	CD45	Leukocyte gate (all white blood cells)

This document (01.0280.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.