PerCP (Peridinin-Chlorophyll-Protein) antibody conjugates are essential tools for multicolor flow cytometry, offering excitation by the 488 nm laser with emission in the far-red region (~675 nm). AAT Bioquest provides a comprehensive selection of PerCP-conjugated primary antibodies targeting key immune cell markers, enabling researchers to build robust multicolor panels for immunophenotyping applications. These conjugates are particularly valuable when combined with FITC and PE conjugates on the same laser line, allowing efficient use of instrument configurations.
PerCP and PerCP Tandem Conjugated CD Antibodies
PerCP-conjugated CD antibodies utilize a natural photosynthetic pigment-protein complex from dinoflagellates, providing bright far-red fluorescence (~675 nm) when excited by the common 488 nm laser. With minimal spectral overlap with FITC and PE channels, PerCP is an excellent third-color choice for basic multicolor immunophenotyping panels. For additional spectral flexibility, PerCP tandem conjugates (such as PerCP-Cy5.5) use fluorescence resonance energy transfer (FRET) to shift emission further into the red region (~695 nm). This extended wavelength provides better separation from PE-based tandems and enables more complex panel designs on single-laser instruments.
Key Features
488 nm laser compatible – Works with standard argon-ion lasers on most flow cytometers
Far-red emission options – PerCP (~675 nm) or tandem conjugates (~695 nm) for flexible panel design
Minimal spectral overlap – Excellent separation from FITC, PE, and PE-based tandems
High brightness & photostability – Reliable signal-to-noise ratio for cell population identification
Validated performance – Extensively tested for T cell, B cell, and myeloid marker detection
ReadiLink™ — High-yield labeling with small molecules such as fluorescent dyes, no purification required
Buccutite™ — Proprietary chemistry optimized for labeling with proteins like HRP, PE, and APC ReadiUse™ — One-step labeling with preactivated dyes and tags, ideal for quick conjugation workflows
This document (01.0284.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.