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Reporter Tag Proteins
Reporter tag proteins and detection reagents are essential tools for protein engineering and analysis. AAT Bioquest provides HIS Lite™ NTA-Ni fluorescent probes for sensitive detection of His-tagged proteins in gels, blots, and live cells, and recombinant firefly luciferase for bioluminescence-based reporter gene assays and ATP quantification.
Product Type | Application | Best For | Key Products |
|---|---|---|---|
Fluorescent His-Tag Probes | Bioconjugation, surface immobilization | SDS-PAGE, Western blot, live cell imaging | HIS Lite™ iFluor® & Cy NTA Complexes |
Recombinant Luciferase | Reporter gene assays, bioluminescence | Gene expression studies, ATP quantification | Firefly Luciferase |
His-Tag Detection
HIS Lite™ NTA-Ni complexes are fluorescent probes designed for specific detection of polyhistidine-tagged proteins in gels, blots, and live cells. These probes feature either Bis-NTA (two NTA groups) or Tris-NTA (three NTA groups) configurations, with Tris-NTA providing higher affinity and more stable binding to His-tags. The chelator versions require addition of nickel before use, while pre-complexed versions are ready-to-use.
Key Features
- Tris-NTA configuration – provides sub-nanomolar affinity for exceptional sensitivity
- iFluor® dye options – offer superior brightness and photostability compared to conventional dyes
- Direct gel staining – eliminates the need for transfer to membranes
- Live cell compatible – for tracking His-tagged proteins in real-time
- Multiple fluorophore options – for multiplexing with other detection methods
Recombinant Luciferase
Firefly luciferase is a widely used bioluminescent reporter enzyme for gene expression studies, promoter analysis, and ATP quantification. The enzyme catalyzes ATP-dependent oxidation of D-luciferin to produce light emission at ~560 nm, providing a sensitive and quantitative readout for reporter gene assays.
Key Features
- Enhanced thermal stability — Improved stability at elevated temperatures compared to native enzyme
- High specific activity — Consistent lot-to-lot performance for reproducible results
- Recombinant production — Reliable supply without dependence on firefly harvesting
- Compatible with Amplite® assay kits — Optimized for use with AAT Bioquest luciferase detection reagents
Applications
- Reporter gene assays for promoter and enhancer studies
- Dual-reporter assay compatibility when used alongside Renilla luciferase systems
- ATP quantification assays
- High-throughput screening
This document (01.0296.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Reporter Tag Proteins