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Resins and Columns
AAT Bioquest offers a comprehensive selection of resins and columns for protein purification, nucleic acid isolation, and buffer exchange applications. The ReadiPrep™ and ReadiUse™ product lines provide reliable, high-quality solutions for common sample preparation workflows. These products are designed for optimal performance, reproducibility, and compatibility with standard laboratory protocols.
Product Group | Example Products |
|---|---|
Protein Purification Resins | Agarose Resins |
Nucleic Acid Purification Columns | Silica Spin Columns |
Buffer Exchange and Desalting Columns | Size Exclusion Columns |
Protein Purification Resins
AAT Bioquest offers ReadiPrep™ affinity resins for efficient protein purification using immobilized metal affinity chromatography (IMAC) and immunoaffinity approaches. These agarose-based resins provide high binding capacity, excellent flow properties, and reproducible protein recovery for both batch and column purification formats.
His-Tagged Protein Purification
The ReadiPrep™ Ni Agarose Fast Flow resin utilizes nickel-charged nitrilotriacetic acid (Ni-NTA) chemistry for highly specific capture of polyhistidine-tagged recombinant proteins. This IMAC approach is widely used for purifying expressed proteins from bacterial, yeast, insect, and mammalian cell lysates with excellent recovery rates.
Key Features
- High binding capacity for His-tagged proteins
- Fast flow agarose matrix for efficient processing
- Compatible with batch, gravity, and FPLC purification methods
- Reproducible recovery with minimal non-specific binding
- Native and denaturing purification conditions supported
Antibody Purification (Protein A/G Resins)
ReadiPrep™ Protein A and Protein G agarose resins are designed for immunoglobulin purification through highly specific interactions with the Fc region of antibodies. Protein A and Protein G have different binding affinities for various IgG subclasses and species, allowing researchers to select the optimal resin for their specific antibody purification needs.
Key Features
- Covalently immobilized Protein A or G on crosslinked beaded agarose
- Excellent binding capacity for high-yield antibody recovery
- Batch and column purification format compatible
- IgG purification from serum, ascites, or cell culture supernatant
- Protein G offers broader species reactivity compared to Protein A
Fig. 1
Protein A agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure. Protein A contains four high-affinity binding sites interacting with the Fc region of IgG-class antibodies from selected mammalian species.
Nucleic Acid Purification Columns
ReadiPrep™ silica-based spin columns provide rapid, efficient purification of plasmid DNA and PCR products using solid-phase extraction. In the presence of chaotropic salts, nucleic acids bind selectively to the silica membrane while contaminants are washed away, yielding high-quality DNA suitable for downstream applications including sequencing, cloning, and transfection.
Key Features
- Silica membrane technology for high-purity nucleic acid isolation
- Fast spin-column format compatible with standard microcentrifuges
- High recovery yields with minimal sample loss
- Transfection-grade DNA from plasmid columns
- QIAquick compatible PCR cleanup columns
Fig. 2
Illustration of solid-phase DNA extraction using silica gel spin columns. In the presence of chaotropes, DNA binds to silica resins while other cellular components are washed away.
Buffer Exchange and Desalting Columns
ReadiUse™ size exclusion chromatography columns enable rapid buffer exchange, desalting, and removal of small molecule contaminants from protein and nucleic acid samples. These pre-packed columns use gel filtration matrices that separate molecules based on size, allowing macromolecules to pass through quickly while retaining salts, dyes, and other low molecular weight substances.
Key Features
- Pre-packed, ready-to-use format saves preparation time
- Bio-Gel P-6 columns ideal for small sample volumes (50-100 µL)
- PD-10 columns (Sephadex G-25) accommodate larger volumes up to 2.5 mL
- Efficient removal of salts, free labels, and reaction byproducts
- Excellent recovery of proteins and nucleic acids
Fig. 3
Prepacked ReadiUse™ Bio-Gel P6 Spin Column. The column was packed with P-6 DG in PBS buffer for sample volume 50~100 uL. The effective exclusion range is approximately 6 kDa, allowing proteins (>15 kDa) to elute in the void volume while retaining small molecules.
This document (01.0344.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.