Name: Advantages and Disadvantages of Cell Proliferation Assays
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Advantages and Disadvantages of Cell Proliferation Assays

Cell Proliferation Assays	Advantages	Disadvantages
BrdU	Direct measurement of DNA synthesis Strong linear correlation with increasing cell numbers Compatible with cell sorters and microscopy	Requires fixation and DNA denaturation Cannot quantify cell number Associated health risks
EdU	Increased selectivity in comparison to BrdU Simple, fast and reliable High sensitivity Very low toxicity Provides multiplexing possibilities	Relatively expensive Limited to cells that can be labeled with EdU
Bucculite™ XdU or FdU	No DNA denaturation needed Does not rely on antibodies Preserves cell morphology, antigen-binding sites, and sample integrity	No major disadvantage
Tetrazolium Salt	Simple and inexpensive Measures metabolic activity of the cells Easy to perform Suitable for high-throughput screening	Metabolic behavior tends to be variable under different cell culture conditions Standards must be built, and are not typically provided Requires use of phenol red free media due to potential interference Not suitable for non-adherent cells
Resazurin	Low toxicity Inexpensive Highly sensitive Great for long-term studies with various cell types Allows for real-time monitoring	Prone to fluorescence interference Data might be affected by cell density and media composition Determining inhibition kinetics and optimizing the procedure remains challenging
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MLA	"Quest Database™ Advantages and Disadvantages of Cell Proliferation Assays." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/data-sets/advantages-and-disadvantages-of-cell-proliferation-assays.
APA	AAT Bioquest, Inc. (2025, December 5). Quest Database™ Advantages and Disadvantages of Cell Proliferation Assays. AAT Bioquest. https://www.aatbio.com/data-sets/advantages-and-disadvantages-of-cell-proliferation-assays.
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