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Advantages, disadvantages and applications of End-Point RT-PCR
End-Point RT-PCR
Advantages
  • Convenient - suitable for analysis of any double-strand DNA sequence
  • Economical - cheaper than using custom designed probes
Disadvantages
  • Poor precision and low sensitivity
  • Short dynamic range < 2 logs
  • Low resolution, size-based discrimination only
  • Not suitable for automation
  • Results are not expressed as numbers
  • Post-PCR processing
Applications
  • Gene expression analysis
  • Cloning
  • Contructing cDNA libraries
  • RNA sequencing
  • ClonMicroarray probe developmenting
  • Species identification

References
This online resource may be cited as follows
MLA
"Quest DatabaseAdvantages, disadvantages and applications of End-Point RT-PCR." AAT Bioquest, Inc.3 Apr2026https://www.aatbio.com/data-sets/advantages-disadvantages-and-applications-of-end-point-rt-pcr.
APA
AAT Bioquest, Inc. (2026April 3). Quest DatabaseAdvantages, disadvantages and applications of End-Point RT-PCR. AAT Bioquest. https://www.aatbio.com/data-sets/advantages-disadvantages-and-applications-of-end-point-rt-pcr.
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