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Common Mammalian Cell Lysis Buffers for General Protein Extraction
Buffer
RIPA Lysis Buffer
NP-40 Lysis Buffer
PurposeRecommended for the extraction of cytoplasmic, nuclear, membrane-bound and mitochondrial proteins. It contains harsh denaturing, ionic detergents and milder non-ionic detergets. Disrupts protein-protein interactions.Recommended for extraction of cytoplasmic and membrane-bound proteins. It is a milder, non-ionic detergent. Proteins retain their native state, and protein-protein interactions are preserved.
Composition
  • 25 mM Tris-HCl, pH 7.6
  • 150 mM NaCl
  • 1% NP-40 (non-ionic deterget)
  • 1% sodium deoxycholate (ionic deterget)
  • 0.1% SDS (ionic deterget)
  • 50 mM Tris, pH 7.4
  • 250 mM NaCl
  • 5 mM EDTA
  • 50 mM NaF
  • 1 mM Na3VO4
  • 1% NP-40 (non-ionic deterget)
  • 0.02% NaN3
Compatible protein assaysBCA assaysBCA assays
Recommended applicationsSDS-PAGE, Western blotELISA, protein electrophoresis, Western blot

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References
This online resource may be cited as follows
MLA
"Quest Database™ Common Mammalian Cell Lysis Buffers for General Protein Extraction." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/data-sets/common-mammalian-cell-lysis-buffers-for-general-protein-extraction.
APA
AAT Bioquest, Inc. (2025, December 5). Quest Database™ Common Mammalian Cell Lysis Buffers for General Protein Extraction. AAT Bioquest. https://www.aatbio.com/data-sets/common-mammalian-cell-lysis-buffers-for-general-protein-extraction.
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