Common Mammalian Cell Lysis Buffers for General Protein Extraction

Name: Common Mammalian Cell Lysis Buffers for General Protein Extraction
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Buffer ▲ ▼	RIPA Lysis Buffer ▲ ▼	NP-40 Lysis Buffer ▲ ▼
Purpose	Recommended for the extraction of cytoplasmic, nuclear, membrane-bound and mitochondrial proteins. It contains harsh denaturing, ionic detergents and milder non-ionic detergets. Disrupts protein-protein interactions.	Recommended for extraction of cytoplasmic and membrane-bound proteins. It is a milder, non-ionic detergent. Proteins retain their native state, and protein-protein interactions are preserved.
Composition	25 mM Tris-HCl, pH 7.6 150 mM NaCl 1% NP-40 (non-ionic deterget) 1% sodium deoxycholate (ionic deterget) 0.1% SDS (ionic deterget)	50 mM Tris, pH 7.4 250 mM NaCl 5 mM EDTA 50 mM NaF 1 mM Na3VO4 1% NP-40 (non-ionic deterget) 0.02% NaN3
Compatible protein assays	BCA assays	BCA assays
Recommended applications	SDS-PAGE, Western blot	ELISA, protein electrophoresis, Western blot

References

MLA	"Quest Database™ Common Mammalian Cell Lysis Buffers for General Protein Extraction." AAT Bioquest, Inc., 19 May. 2025, https://www.aatbio.com/data-sets/common-mammalian-cell-lysis-buffers-for-general-protein-extraction.
APA	AAT Bioquest, Inc. (2025, May 19). Quest Database™ Common Mammalian Cell Lysis Buffers for General Protein Extraction. AAT Bioquest. https://www.aatbio.com/data-sets/common-mammalian-cell-lysis-buffers-for-general-protein-extraction.
BibTeX	EndNote	RefMan