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Common Recipe for Making an SDS-PAGE Gel
| Reagents ▲ ▼ | 6% Stacking gel ▲ ▼ | 10% Separating gel ▲ ▼ |
| ddH2O | 2 mL | 3 mL |
| 1 M Tris-HCl | 400 uL pH 6.7 | 2.1 mL pH 8.9 |
| 30% Acrylamide/Bis- Acrylamide | 600 uL | 2.8 mL |
| 10% SDS | 36 uL | 80 uL |
| 10% APS (Ammonium Persulfate) | 24 uL | 50 uL |
| TEMED (TetraMethylEthyleneDiamine) | 4 uL | 6 uL |
References
This online tool may be cited as follows
MLA | "Quest Database™ Common Recipe for Making an SDS-PAGE Gel." AAT Bioquest, Inc., 30 Aug. 2025, https://www.aatbio.com/data-sets/common-recipe-for-making-an-sds-page-gel. | |
APA | AAT Bioquest, Inc. (2025, August 30). Quest Database™ Common Recipe for Making an SDS-PAGE Gel. AAT Bioquest. https://www.aatbio.com/data-sets/common-recipe-for-making-an-sds-page-gel. | |
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