Name: Comparison of MTT and ATP-based assays commonly used in cell viability and proliferation studies.
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Comparison of MTT and ATP-based assays commonly used in cell viability and proliferation studies.

Aspect	MTT Assay (2D)	MTT Assay (3D)	ATP-based Assay (2D)	ATP-based Assay (3D)
Principle	Measures mitochondrial activity (metabolic activity)	Measures mitochondrial activity (metabolic activity)	Measures ATP production (cell viability)	Measures ATP production (cell viability)
Advantages	Widely used, well-established Cost-effective Simple protocol Compatible with various cell types Measures metabolic changes	Applicable to some 3D cultures Suitable for simple 3D models Can capture metabolic changes Provides insights into 3D-specific effects Non-destructive to some 3D cultures	Direct measurement of cell viability Sensitive and rapid Compatible with various cell types Measures ATP production in real-time Detects changes in real-time, indicating viability	Direct measurement of cell viability Suitable for some 3D models Minimal interference from dead cells Suitable for high-throughput screening Provides insights into 3D-specific effects
Disadvantages	Indirect measurement of viability May yield false positives in some cases Requires solubilization step for quantification Requires solubilization step for quantification	Limited applicability to complex 3D models May require additional assays for accuracy May underestimate viability in certain cases Difficulties in uniform compound exposure	Requires ATP extraction or lysis Limited sensitivity in low ATP conditions More expensive reagents May not distinguish between viable and stressed cells	Requires ATP extraction or lysis Sensitivity to environmental factors More expensive reagents May not distinguish between viable and stressed cells
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MLA	"Quest Database™ Comparison of MTT and ATP-based assays commonly used in cell viability and proliferation studies.." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/data-sets/comparison-of-mtt-and-atp-based-assays-commonly-used-in-cell-viability-and-proliferation-studies.
APA	AAT Bioquest, Inc. (2025, December 5). Quest Database™ Comparison of MTT and ATP-based assays commonly used in cell viability and proliferation studies.. AAT Bioquest. https://www.aatbio.com/data-sets/comparison-of-mtt-and-atp-based-assays-commonly-used-in-cell-viability-and-proliferation-studies.
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