Difference Between qPCR and ddPCR

Name: Difference Between qPCR and ddPCR
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Basis of Differentiation ▲ ▼	qPCR ▲ ▼	ddPCR ▲ ▼
Principle	qPCR measures PCR amplification as it happens	ddPCR measures the fraction of positive and negative duplicates to determine absolute copies
Sensitivity	Moderate: detection limit is from 1-10	High: detection limit as low as 1 in 2000
Methods of data collection	Targets with unknown quantity are compared to standard curve with known quantities	PCR reaction is split into thousands of separate real-time PCR reactions before amplification, and data is only obtained at the end-point
Quantification type (Relative or absolute)	Both, but a standard curve with known absolute quantities of the target is necessary for absolute quantification	Absolute only, ddPCR gives an absolute count of target DNA copies per input sample (no standard curve necessary)
Time established	Established technology	Emerging technology

References

MLA	"Quest Database™ Difference Between qPCR and ddPCR." AAT Bioquest, Inc., 27 Aug. 2025, https://www.aatbio.com/data-sets/difference-between-qpcr-and-ddpcr.
APA	AAT Bioquest, Inc. (2025, August 27). Quest Database™ Difference Between qPCR and ddPCR. AAT Bioquest. https://www.aatbio.com/data-sets/difference-between-qpcr-and-ddpcr.
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